Biolegend, 646851, Purified anti-Histone H3 (C-terminus) Recombinant Antibody, 25μg

Histone proteins are classified into core histones (H2A, H2B, H3, H4) and linker histones (H1, H5). Core histones form an octamer, which contains two H2A-H2B dimers and one H3-H4 tetramer. Core histones are predominantly globular except for the unstructured N-terminal tails. Posttranslational modifications, such as acetylation, methylation, phosphorylation, ubiquitination, SUMOylation and ADP-ribosylation occur in histone tails. Histone modifications induce changes of chromatin structure and thereby affect the accessibility of transcription factors, nuclear proteins and enzymes to genomic DNA, resulting in gene activation or repression. It is known that histone modifications play critical roles in DNA repair, DNA replication, transcription regulation, alternative splicing and chromosome condensation and some diseases including autoimmune diseases and cancers.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Recombinant
Host Species: Mouse
Immunogen: This recombinant monoclonal antibody was raised against a peptide sequence derived from the C-terminal domain of human Histone H3 protein.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P, ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.0125 - 0.025 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.25 - 1.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 0.5 - 1.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For immunohistochemistry use on formalin-fixed paraffin-embedded tissue (IHC-P), either antigen retrieval with Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704) is recommended. For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with either of the following: Fixation buffer (Cat. No. 420801) followed by 0.5 % Triton X-100 Fixation buffer (Cat. No. 420801) followed by 100% ice-cold methanol 100% ice-cold methanol only
RRID: AB_3675179 (BioLegend Cat. No. 646851) AB_3675179 (BioLegend Cat. No. 646852)
Structure: Histone proteins H3 and H4 bind to form a H3-H4 dimer, two of these H3-H4 dimers combine to form a tetramer. This tetramer further combines with two H2a-H2b dimers to form the compact Histone octamer core.
Distribution: Nucleus
Function: Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
Interaction: Rsk-2, MSK1, GCN5 family of HATs, HDACs, PARP, SET7/9, and CARM1
Biology Area: Cell Biology, Chromatin Remodeling/Epigenetics, Neuroscience, Transcription Factors
Antigen References: Chen HM, et al. 2017. Chinese Journal of Hematology 38:744-78.
Gene ID: 8350
UniProt: View information about Histone H3 on UniProt.org
Clone: 1B1-B2.RecM1
Regulatory Status: RUO
Other Names: He clustered Histone 1, Histone H3.1, histone H3/a, histone 1, H3a, H3 histone family, member A, H3/A, H3FA
Isotype: Mouse IgG1, κ