Biolegend, 647051, Purified anti-SUZ12 Antibody, 25μg

The SUZ12 protein is a key component of the Polycomb Repressive Complex 2 (PRC2), a major player in gene silencing through histone modification. SUZ12 is essential for the PRC2 complex's ability to methylate histone H3 at lysine 27 (H3K27me3). This methylation marks chromatin for repression, leading to transcriptional silencing of target genes. By contributing to the formation of H3K27me3 marks, SUZ12 helps maintain the repression of genes involved in various cellular processes including development, differentiation, and cell cycle regulation. SUZ12 and the PRC2 complex are crucial for normal embryonic development. They regulate the expression of genes necessary for maintaining pluripotency in embryonic stem cells and for proper lineage commitment during development. Abnormal SUZ12 expression or function are linked to various cancers. SUZ12, as part of PRC2, can act as an oncogene or tumor suppressor, depending on the context. Its deregulation can lead to inappropriate gene silencing or activation, contributing to tumorigenesis.
25μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human SUZ12
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 0.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody (clone W22172C) is not suitable for immunocytochemistry (ICC) or immunohistochemistry (IHC).
RRID: AB_3662328 (BioLegend Cat. No. 647051) AB_3662328 (BioLegend Cat. No. 647052)
Structure: Contain a C2H2-type Zinc finger domain
Distribution: Located in the nucleus, SUZ12 is overexpressed in breast and colon cancer
Function: A component of Polycomb Repressive Complex 2 (PRC2) which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3
Interaction: Part of Polycomb Repressive Complex 2 (PRC2); interacts with EED, EZH1, EZH2, JARD2
Biology Area: Chromatin Remodeling/Epigenetics
Antigen References: Pasini D, et al. 2004. EMBO J. 23:4061-71. Cao R, et al. 2004. Mol Cell. 2004.15:57-67. Pasini D, et al. 2007. Mol Cell Biol. 2007. 27:3769–3779. Lee S-R, et al. 2015. Clin Cancer Res. 21:5391-403. Højfeldt JW, et al. 2018. Nat Struct Mol. Biol. 25:225-232.
Gene ID: 23512
UniProt: View information about SUZ12 on UniProt.org
Clone: W22172F
Regulatory Status: RUO
Other Names: SUZ12 Polycomb Repressive Complex 2 Subunit, Polycomb protein SUZ12, Chromatin precipitated E2F target 9 protein, ChET 9 protein, CHET9, Joined To JAZF1 Protein, JJAZ1, Suppressor Of Zeste 12 Protein Homolog
Isotype: Rat IgG2a, κ