Biolegend, 647201, Purified anti-Caspase-11 Antibody, 25μg

Murine caspase-11 is an upstream protease of caspase-1 and interacts with the proform of caspase-1 which in turn activates IL-1 beta. Caspase-11 is not constitutively expressed in most cell types. Its expression can be upregulated by LPS, IFN-gamma, and other pro-inflammatory stimuli. NF-κB and STAT1 were reported to be involved in the activation. Caspase-11 knock -out mice were resistant to endotoxin shock and do not produce IL-1 after stimulation with LPS. Their production of IFN-gamma was also deficient.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: His tagged bacterial fusion protein of p 30 of caspase 11
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): 1 to 2 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Schmidt RL, et al. 2012. PLoS One. 7:e45186. PubMed.
Product Citations: Khameneh H, et al. 2011. PLoS One. 6:e23025. PubMed Schmidt R, Lenz L 2012. PLoS One. 7:e45186. PubMed Min L, et al. 2012. J Immunol. 188:1789. PubMed Evavold CL, et al. 2021. Cell. 184(17):4495-4511.e19. PubMed
RRID: AB_1937283 (BioLegend Cat. No. 647201) AB_1937281 (BioLegend Cat. No. 647202)
Structure: This murine caspase 11 has pro-enzymes a 38 and 43 kD. The activated form consists of p30 and p10.
Distribution: Not constitutively expressed in most cell types, but can be induced by different pro-inflmmatory stimuli including LPS.
Function: Essential for the activation of caspase-1, thus involved in the maturation of IL-1beta and IL-18. Both are important mediators of inflammation.
Interaction: Caspase-1
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience
Antigen References: 1. Lee J et al. 2001. J. Biol. Chem. 276:329562. Schauvliege R et al. 2002. J. Biol. Chem. 277:416243. Wang S et al. 1998. Cell 92:501
Gene ID: 201161
UniProt: View information about Caspase-11 on UniProt.org
Clone: Cas11.17D9
Regulatory Status: RUO
Other Names: Casp11
Isotype: Rat IgG2a