Biolegend, 647401, Purified anti-mMCP-8 Antibody, 25μg

mMCP-8 and its rat homologues, rMCP-8, -9, and -10, form a new group of mast cell/basophil proteases, which are more closely related to the T-cell granzymes and neutrophil cathepsin G than to the mast cell tryptases and chymases. mMCP-8 showed a high degree of homology with mouse granzyme B in the critical regions for determining substrate cleavage specificity. It preferentially cleaves after Asp residues. mMCP-8 mRNA is highly expressed in a mouse connective tissue MC-like tumor line. However, it could not be detected in mouse liver, intestine, lung or ears, indicating very low expression in normal tissues. A strong increase in mMCP-8 levels in the lungs can be detected in infected animals.
25μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant GST-mouse mMCP-8 fusion protein expressed in E.coli
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The optimal concentration should be determined by titration for each individual assay of interest.
Application References(PubMed link indicates BioLegend citation): Ugajin T, et al. 2009. J. Leukoc. Biol. 86:1417. (IHC) Uto-Konomai A, et al. 2012. PLoS One. 7:e40343. PubMed Morshed M, et al. 2014. J Immunol. 192:5314. PubMed Schwartz C, et al. 2014. J Immunol. 193:3590. PubMed Schwartz C, et al. 2014. PNAS. 111:5169. PubMed
Product Citations: Schwartz C, et al. 2014. J Immunol. 193:3590. PubMed Morshed M, et al. 2014. J Immunol. 192:5314. PubMed Piliponsky AM, et al. 2019. Nat Immunol. 0.922916667. PubMed Webb LM, et al. 2019. J Exp Med. 216:1268. PubMed Monte L, et al. 2016. Cancer Res . 76: 1792-1803. PubMed Sicklinger F, et al. 2021. J Clin Invest. 131:. PubMed Schwartz C, et al. 2014. Proc Natl Acad Sci U S A. 111:5169. PubMed Imai Y, et al. 2017. Sci Rep. 10.1038/s41598-017-10227-y. PubMed Pattabiraman G, et al. 2021. Am J Physiol Renal Physiol. . PubMed
RRID: AB_2069309 (BioLegend Cat. No. 647401) AB_2290790 (BioLegend Cat. No. 647402)
Structure: A 247-aa polypeptide including a signal sequence of 18 aa followed by a 2-aa activation peptide (Gly-Glu) and a mature protease of 227 aa
Distribution: mMCP-8 was initially isolated as a cDNA from a mouse mast cell line, but has recently been found to be expressed primarily by mouse basophils.
Cell Type: Basophils, Mast cells
Biology Area: Cell Biology, Immunology
Antigen References: 1. Lutzelschwab C, et al. 1998. Eur. J. Immunol. 28:1022. 2. Lunderius C, et al. 2001. Immunogenetics 53:225.
Gene ID: 4179
UniProt: View information about mMCP-8 on UniProt.org
Clone: TUG8
Regulatory Status: RUO
Other Names: Mouse Mast Cell Protease 8, Mcpt8
Isotype: Rat IgG2a, κ