Biolegend, 647653, Ultra-LEAF™ purified anti-human PLGF Antibody, 100μg

Placenta growth factor (PLGF) was initially cloned form a term placenta cDNA library. PLGF is a dimeric glycoprotein with 53% homology to VEGF. As a result of alternative splicing, four isoforms for PLGF has been described, PLGF-1, PLGF-2, PLGF-3, and PLGF-4.  PLGF-2 binds to heparin with high affinity, whereas neither PLGF-1, PLGF-3, nor PLGF-4 bind heparin. PLGF-2 is the only isoform in mouse. PLGF binds to VEGFRI but not to VEGFRII; also, PLGF binds to sVEGF1. PLGF plays a key role in the maternal vascular function during pregnancy. Low levels of PLGF have been associated with patients experiencing preeclampsia and delivering small-for-gestational age (SGA) neonates. High expression of PLGF has been associated to colorectal cancer (CRC), and its expression in CRC tissues correlates with high levels of MMP-9 and VEGF1. PLGF has been shown to increase tumor cell migration in lung cancer, leukemia, and melanoma cells. In colorectal cancer, PLGF induces tumor cell migration and invasion through upregulation of MMP9. PLGF is expressed in the majority of medulloblastomas, and high expression of PLGF receptor neuropilin 1 (Nrp1) correlates with poor survival in patients. In a mouse model, PLGF/Nrp1 blockade provided direct antitumor effects in vivo , medulloblastoma regression, decreased metastasis, and increased survival.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human PLGF
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/μg of the protein (less than 0.001 ng/μg of the protein) as determined by the LAL test
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Block - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by blocking the binding of 0.25 µg/mL recombinant human VEGFR1 (Cat. No. 555804) to immobilized 0.5 µg/mL recombinant human PLGF (Cat. No. 590704). ND50 range: 0.06 - 0.35 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_3662330 (BioLegend Cat. No. 647653) AB_3662330 (BioLegend Cat. No. 647654)
Structure: Homodimer
Distribution: Vascular endothelial cells, smooth muscle cells, keratinocytes, hematopoitic cells, retinal pigment epithelial cells, tumor cells, and cerebellar stroma cells
Function: PLGF is an angiogenic factor and induces proliferation and migration of endothelial cells. It stimulates endothelial cells and synergistically amplifies the action of VEGF. Soluble VEGFR1 (sFLT1) can regulate the accessibility of PLGF.
Interaction: Endothelial cells, Macrophages, and trophoblast
Ligand/Receptor: VEGFR1 (FLT-1), and neuropilin 1 (Nrp1)
Cell Type: Hematopoietic stem and progenitors
Biology Area: Cell Biology, Signal Transduction, Stem Cells
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: Maglione D, et al. 1991. Proc Natl Acad Sci USA. 88:9267-71. Carmeliet P, et al. 2001. Nat Med. 7:575-83. Casalou C, et al. 2007. Leukemia. 21:1590-4. Marcellini M, et al. 2006. Am J Pathol. 169:643-54. Yao J, et al. 2011. Proc Natl Acad Sci USA. 108:11590. Cai J, et al. 2011. PLoS One. 6:e18076. Sibiude J, et al. 2012. PLoS One. 7:e50208. Snuderl M, et al. 2013. Cell. 152:1065-76.
Gene ID: 5228
UniProt: View information about PLGF on UniProt.org
Clone: W22016A
Regulatory Status: RUO
Other Names: Placental growth factor 1 (PGF1)
Isotype: Rat IgG2a, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.