Biolegend, 647854, Ultra-LEAF™ Purified anti-human CHI3L1 (YKL-40) Recombinant Antibody, 1mg

Chitinase-3-like protein 1 (CHI3L1), also known as YKL-40, is a secreted member of the glycoside hydrolase family that lacks chitinase activity and is produced by activated macrophages. CHI3L1 is expressed in a variety of cancers, where it plays roles in cell proliferation, invasiveness and cell-matrix interactions. CHI3L1 activates the IL-13Rα2 receptor which leads to ERK phosphorylation and increased proliferation. Neutralizing antibodies against CHI3L1 can block tumor metastasis in animal models. CHI3L1 is also induced at sites of inflammation, infection, and tissue remodeling, where it downregulates the production of IFN-γ in T lymphocytes and promotes Th2 and IL-13-driven inflammatory responses. The measurement of CHI3L1 levels in cerebrospinal fluid or blood may serve as a biomarker of Alzheimer’s disease.
1mg
Verified Reactivity: Human
Antibody Type: Recombinant
Host Species: Mouse
Immunogen: Human Chitinase 3-like 1 protein
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Block - Quality tested ICFC, ICC - Verified
Recommended Usage: Ultra-LEAF Purified anti-human CHI3L1 (YKL-40) recombinant antibody inhibits CHI3L1-mediated cell migration and pERK signaling. For blocking of CHI3L1-induced cell migration, a concentration range of 0.06 - 2.0 μg/mL is recommended. For blocking of CHI3L1- induced signaling, a concentration range of 2.0 - 5.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 2.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: Ultra-LEAF Purified anti-human CHI3L1 (YKL-40) recombinant antibody inhibits CHI3L1-mediated cell migration and pERK signaling.
RRID: AB_3717108 (BioLegend Cat. No. 647853) AB_3717108 (BioLegend Cat. No. 647854)
Structure: Secreted glycoprotein, glycoside hydrolase family
Distribution: Cytoplasm, extracellular space
Function: Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity.
Interaction: LEG3
Ligand/Receptor: IL13Ra2
Cell Type: Endothelial cells, Granulocytes, Leukemia, Macrophages, Neurons
Biology Area: Cancer Biomarkers, Cell Motility/Cytoskeleton/Structure, Cell Proliferation and Viability
Molecular Family: Enzymes and Regulators
Antigen References: Faibish M, et al. 2011. Mol Cancer Ther. 10:742-751. Muszynski P, et al. 2017. Curr Neuropharmacol. 15:906-917. Francescone R, et al. 2011. J Biol Chem. 286:15332-15343. Hong, D. E., et al. 2022. Molecules. 28:329. Yu, J.E., et al. 2021. Mol. Onc. 16:2214-2234.
Gene ID: 1116
UniProt: View information about CHI3L1 on UniProt.org
Clone: A19120F.Rec
Regulatory Status: RUO
Other Names: YKL-40, Chitinase-3-like protein 1
Isotype: Mouse IgG1, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.