Biolegend, 648452, Purified anti-SDHA Antibody, 100μg

Succinate dehydrogenase (SDH), commonly known as respiratory complex II, is an important enzyme of mitochondria in the citric acid cycle and electron transport chain, and consists of four subunits SDHA, SDHB, SDHC, and SDHD. SDHA, like other SDHx genes, can function as a tumor suppressor gene. Recently, studies have indicated that mutations in SDHA contribute to various disorders such as hereditary pheochromocytoma-paraganglioma (PPGL) syndrome, mitochondrial complex II deficiency, gastrointestinal stromal tumors, Leigh syndrome, dilated cardiomyopathy, and neuroendocrine tumors.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human SDHA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P, ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: This antibody has been tested in knockout/knockdown systems for WB and ICC. This antibody (clone W23126E) does not cross-react with mouse SDHA on Western Blot (WB). For immunocytochemistry (ICC), we recommend fixation with 4% PFA fixation followed by permeabilization with 0.5% Triton X-100 or ice cold-methanol, or ice-cold methanol fixation. For immunohistochemistry on FFPE tissues (IHC-P), antigen retrieval with Citrate Buffer (Cat. No. 420901) or Tris-EDTA pH 9.0 (Cat. No. 422704) is recommended.
RRID: AB_3717114 (BioLegend Cat. No. 648451) AB_3717114 (BioLegend Cat. No. 648452)
Structure: SDHA is a 664 amino acid protein with a predicted molecular weight of 73 kD.
Distribution: Membrane and mitochondrion inner membrane
Function: An important enzyme of mitochondria in the citric acid cycle and electron transport chain, tumor suppressor gene, prognostic marker
Interaction: Interacts with SDHAF2/SDH5, TRAP1, LACC1.
Biology Area: Cardiovascular Biology, Cell Biology, Cell Death, Cell Proliferation and Viability, Neurodegeneration, Neuroscience
Molecular Family: Mitochondrial Markers, Tumor Suppressors
Antigen References: Oyedotun KS, et al. 2004. J Biol Chem. 279: 9424. Rutter J, et al.2011. Mitochondrion.10:393. Burnichon N. 2010. Hum Mol Genet.19: 3011. Benchoua A, et al. 2006. Mol Biol Cell. 17:1652.
Gene ID: 6389
UniProt: View information about SDHA on UniProt.org
Clone: W23126E
Regulatory Status: RUO
Other Names: succinate dehydrogenase complex flavoprotein subunit A , CMD1GG , FP , PGL5 , SDH1 , SDH2 , SDHF
Isotype: Rat IgG2a, κ