Biolegend, 648811, Purified anti-human CD279 (PD-1) Antibody, 100μg

Programmed cell death 1 (PD-1), also known as CD279, is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune disease. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 (B7-H1) and PDL2 (B7-DC) are ligands of CD279 (PD-1) and are members of the B7 gene family. Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Interaction of CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human PD-1 Recombinant Protein (His & Fc Tag)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: Direct ELISA - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.
Additional Product Notes: This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone A20074H) could function as a capture antibody when paired with biotin anti-human PD-1 antibody (Cat. No. 621627) (clone A17188B) as the detection antibody and using Recombinant Human PD-1 (carrier-free) protein as the assay standard.
RRID: AB_3717175 (BioLegend Cat. No. 648811)
Structure: Immunoglobulin superfamily
Distribution: Transiently expressed on CD4- CD8- thymocytes; upregulated in thymocytes and splenic T and B lymphocytes; expressed on activated myeloid cells
Function: Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self
Interaction: APC, monocytes, dendritic cells, stromal cell, cancer cells
Ligand/Receptor: B7-H1 (also known as PD-L1) and B7-DC (PD-L2)
Cell Type: Lymphocytes, T cells
Biology Area: Cell Biology, Immuno-Oncology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: Ishida Y, et al. 1992. EMBO J. 11:3887. Fife BT, et al. 2009. Nature Immunol. 10:1185-92. Freeman GJ, et al. 2000. J Exp Med. 192:1027. Latchman Y, et al. 2001. Nat Immunol. 2001 2:261. Iwai Y, et al. 2002. Proc Natl Acad Sci USA. 99:12293-7. Dai S, et al. 2014. Cell Immunol. 290:72. Tan S, et al. 2017. Nature Commun. 8:14369.
Gene ID: 5133
UniProt: View information about CD279 on UniProt.org
Clone: A20074H
Regulatory Status: RUO
Other Names: Programmed cell death protein 1, Protein PD-1, hPD-1, CD279
Isotype: Rat IgG2a, κ