Biolegend, 648831, Purified anti-COUP-TFI (NR2F1) Antibody, 100μg

COUP-TFI (Chicken Ovalbumin Upstream Promoter-Transcription Factor I), also known as NR2F1, is a nuclear receptor and transcription factor that plays a pivotal role in neurodevelopment, cell differentiation, and gene regulation. It belongs to the steroid/thyroid hormone receptor superfamily and functions primarily by binding to specific DNA motifs (such as 5'-AGGTCA-3') to regulate gene expression either as a repressor or activator. COUP-TFI is essential for proper cortical development, neuronal migration, and axonal guidance, particularly in the central nervous system. Its expression is tightly regulated during embryogenesis and is critical for establishing regional identity in the developing brain. Dysregulation or mutations in the NR2F1 gene are associated with Bosch-Boonstra-Schaaf optic atrophy syndrome (BBSOAS), a rare neurodevelopmental disorder characterized by intellectual disability, optic nerve atrophy, and developmental delays. Additionally, aberrant COUP-TFI activity has been implicated in various cancers and metabolic disorders.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of Human COUP-TFI protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_3720544 (BioLegend Cat. No. 648830) AB_3720544 (BioLegend Cat. No. 648831)
Structure: The COUP-TFI protein consists of 423 amino acid and has a molecular weight of approximately 46.2 kD
Distribution: Nuclear
Interaction: CBP, SRC-1, Specific DNA motifs, FABP7
Cell Type: Endothelial cells, Epithelial cells, Monocytes, Neurons, Neutrophils, NK cells, T cells
Biology Area: Cancer Biomarkers, Cell Biology, Neurodegeneration, Neuroscience, Transcription Factors
Antigen References: Wu X, et al. 2022. Neurosci Bull. 38:505. Feng J, et al. 2021. Sci Adv. 7:eabf6808. Bonzano S, et al. 2018. Cell Rep. 24:329. Yang X, et al. 2017. Curr Top Dev Biol. 125:275.
Gene ID: 7025
UniProt: View information about COUP-TFI on UniProt.org
Clone: W23102A
Regulatory Status: RUO
Other Names: NR2F1, EAR-3, TFCOUP1, COUP transcription factor 1
Isotype: Rat IgG2a, κ