Biolegend, 648838, Purified anti-Annexin A2, 25μg

Annexin A2 (ANXA2), a member of the annexin family of calcium-dependent phospholipid-binding proteins. Annexin A2 contains a conserved core domain with four annexin repeats that facilitate calcium-dependent binding to negatively charged phospholipids. This protein plays roles in diverse cellular processes, including membrane trafficking, cytoskeletal rearrangement, fibrinolysis, inflammation, and cellular signal transduction. Annexin A2 contributes to the regulation of membrane dynamics and endocytosis by binding to negatively charged phospholipids in a calcium-dependent manner, facilitating vesicle aggregation, fusion, and cytoskeletal anchoring. It also supports actin filament organization, thereby influencing cellular shape, motility, and adhesion. Annexin A2 is involved in exocytosis and plays an essential role in the trafficking of proteins to and from the cell membrane. ANXA2 typically exists as a monomer or as a heterotetrameric complex with S100A10 (also known as p11), forming the Annexin A2/S100A10 heterotetramer (A2t). This complex is crucial for extracellular and membrane-associated functions. The A2t complex acts as a co-receptor for tissue plasminogen activator (tPA) and plasminogen on endothelial cell surfaces, promoting the localized generation of plasmin, a key enzyme involved in clot breakdown and extracellular matrix remodeling. Dysregulation of Annexin A2 expression or localization has been associated with various diseases. In cancer, Annexin A2 is frequently overexpressed and promotes tumorigenesis by enhancing cell proliferation, invasion, angiogenesis, and metastasis. It has been identified as a marker of poor prognosis in cancers such as breast, pancreatic, and lung cancer. Annexin A2 facilitates cancer cell migration through actin remodeling and matrix degradation, and its cell surface expression supports plasmin-mediated extracellular matrix breakdown, aiding invasion.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant full length human Annexin A2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P, ICC, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry, a concentration range of 2.5 - 10.0 µg/mL is suggested. For immunohistochemistry, a concentration range of 1.25 - 10.0 µg/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with either of the following: -Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol -100% ice-cold methanol only For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either of the following: -Citrate Buffer, 10X (Cat. No. 420902) -Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704). For use in intracellular flow cytometry (ICFC), it is recommended to fix/permeabilize with True-Phos™ Perm Buffer (Cat. No. 425401). It is not recommended to perform fixation/permeabilization with either of the following: - Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) -True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401)
RRID: AB_3717232 (BioLegend Cat. No. 648838) AB_3717232 (BioLegend Cat. No. 648839)
Structure: Annexin A2 is a 339 amino acid length protein with a predicted molecular weight of 38.6 kD.
Distribution: Cytoplasm, cell surface, and extracellular space
Interaction: S100A10
Cell Type: Endothelial cells, Langerhans cells, Macrophages, Monocytes
Biology Area: Adaptive Immunity, Apoptosis/Tumor Suppressors/Cell Death, Cell Motility/Cytoskeleton/Structure, Cell Proliferation and Viability, Innate Immunity, Signal Transduction
Antigen References: Pomay-Penabad Z, et al. 2009. Blood. 114(14):3074-83. Zhai H, et al. 2011. J. Neurosci. 31(40):14346-60. Lokman NA, et al. 2011. Cancer Microenviron. 4(2):199-208. Christensen MV, et al. 2018. Int. J. Oncol. 52(1):5-18.
Gene ID: 302
UniProt: View information about Annexin A2 on UniProt.org
Clone: W19153A
Regulatory Status: RUO
Other Names: Annexin II, Annexin-2, ANXA2, Calpactin I heavy chain, Calpactin-1 heavy chain
Isotype: Rat IgG2a, κ