Biolegend, 648842, Purified anti-Estrogen Receptor alpha Antibody, 25μg

Estrogen receptor alpha is a nuclear hormone receptor that functions as a transcription factor activated by the hormone estrogen. Upon binding estrogen, ESR1 translocates to the nucleus where it binds to estrogen response elements in the DNA to regulate gene expression. It plays a crucial role in the development and maintenance of reproductive tissues, as well as in bone density, cardiovascular function, and behavior. ESR1 is also a key player in the pathogenesis of hormone-sensitive cancers such as breast, cervical and uterine cancer, where it influences tumor growth and progression.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant protein corresponding to a fragment of the N-terminus of human Estrogen Receptor alpha, including the DNA binding domain.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.25 - 0.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704). Tris-EDTA pH 9.0 may result in slightly brighter signal.
RRID: AB_3720561 (BioLegend Cat. No. 648842) AB_3720561 (BioLegend Cat. No. 648843)
Structure: Estrogen receptor alpha is a 595 amino acid protein with a predicted molecular weight of 66.2 kD.
Distribution: Tissue distribution: reproductive tissues, bone, endothelial cells and vascular smooth muscle cells, liver, certain brain regions such as the hypothalamus and hippocampus Cellular distribution: cytoplasmic, nuclear upon estrogen binding
Function: Nuclear hormone receptor
Interaction: Binds to estrogen-responsive elements in DNA Interacts with activators (e.g. SRC family members, CBP and P300), repressors (e.g. NCOR2, SMRT), and other transcription factors (e.g. SP1, AP1). Phosphorylated by multiple kinases including AKT and MAPK pathway proteins.
Ligand/Receptor: Endogenous estrogens including estradiol (17β-estradiol/E2), estrone (E1) and estriol (E3)
Cell Type: Mature Neurons
Biology Area: Cell Biology, Stem Cells
Molecular Family: Neurotransmitter Receptor Trafficking/Endocytosis, Scaffold Proteins
Antigen References: Schultz J, et al.2002. J Biol Chem. 277:8702. Zivadinovic D, Watson CS 2005. Breast Cancer Res. 7:R310 Wang Y, et al. 2025. Histopathology. 87(1):3-17.
Gene ID: 2099
UniProt: View information about Estrogen Receptor alpha on UniProt.org
Clone: W23263D
Regulatory Status: RUO
Other Names: Estrogen recepto alpha (ERα, ESRA, ESR1), Steroid homone receptor ERR1, Estrogen
Isotype: Rat IgG2a, κ