Biolegend, 648856, Ultra-LEAF™ Purified anti-human AXL Antibody, 100μg

AXL is a receptor tyrosine kinase member of the TAM family that includes Tyro3, AXL, and Mer. The members of this family are type I receptors characterized by two immunoglobulin (Ig)-like and fibronectin III (FNIII) domains in the extracellular domain, a transmembrane domain, and a kinase intracellular domain. The TAM receptors have common ligands, such as GAS-6 and protein S. GAS-6 binds to the three receptors, meanwhile protein S binds only to Tyro3 and Mer. GAS-6 has the highest affinity for AXL, compared to Tyro3 and Mer. AXL is activated by different mechanisms: Gas-6-dependent dimerization, ligand-independent dimerization, and heteromeric dimerization with different receptors that are not members of the TAM family, such as IFNAR, EGFR, PDGFR, and FLT3. Soluble forms of AXL are present in human and murine plasma. They derived of the proteolytic cleavage of the extracellular domain. Soluble forms also bind to GAS-6, acting as inhibitors of AXL-mediated cell activation. AXL is overexpressed in many cancers, such as lung, breast, colon, gastric, ovarian, prostate, thyroid, myeloid leukemia, and chronic lymphocytic leukemia to mention some, and its expression has been associated with poor prognosis.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human AXL extracellular domain (R&D Systems 10448-AL)
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Block - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by blocking the binding of 15 ng/mL of Human AXL-Fc Chimera (Cat. No. 790902) to its ligand Human GAS6 (Cat. No. 796806), immobilized at 2 μg/mL. ND50 range: 6.0 – 56.0 ng/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_3720580 (BioLegend Cat. No. 648856) AB_3720580 (BioLegend Cat. No. 648857)
Structure: Disulfide-linked homodimer
Distribution: Ubiquitously expressed at high levels in platelets, monocytes/macrophages, endothelial cells, mesenchymal, epithelial, and hematopoietic cell lines.
Function: Cellular growth, survival, and differentiation. Promotes tumor cell metastasis, therapeutic resistance, and fosters disease progression.
Interaction: Dendritic cells, Endothelial cells, Epithelial cells, Macrophages, Mesenchymal cells, Monocytes, NK cells, Platelets
Ligand/Receptor: GAS-6
Cell Type: Dendritic cells, NK cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cardiovascular Biology, Cell Death, Cell Proliferation and Viability, Immuno-Oncology, Signal Transduction
Molecular Family: Cytokine/Chemokine Receptors, Cytokines/Chemokines
Antigen References: O'Bryan JP, et al. 1991. Mol. Cell. Biol. 11:5016. Angelillo-Scherrer A, et al. 2001. Nat. Med. 7:215. Budagian V, et al. 2005. Mol. Cell. Biol. 25:9324. Schmidt T, et al. 2012. Cell. Mol. Life Sci. 69:1391. Wu X, et al. 2014. Oncotarget. 5:9546. Axelrod H, Pienta KJ, 2014. Oncotarget. 5:8818.
Gene ID: NA
UniProt: View information about AXL on UniProt.org
Clone: S21005F
Regulatory Status: RUO
Other Names: AXL Receptor Tyrosine Kinase, Tyrosine-Protein Kinase Receptor UFO, AXL Oncogene, AXL Transforming Sequence/Gene, Ty
Isotype: Mouse IgG2a, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.