Biolegend, 648896, Purified anti-KDM3B (JMJD1B) Antibody, 25μg

Lysine demethylase 3B (KDM3B), also known as JHDM2B or JMJD1B, is a member of the Jumonji C (JmjC) domain-containing histone demethylase family, which participates in the epigenetic regulation of gene expression. KDM3B specifically catalyzes the demethylation of mono- and di-methylated lysine 9 on histone H3 (H3K9me1/2), a repressive chromatin mark, thereby promoting transcriptional activation. KDM3B interacts with other chromatin remodelers, transcription factors, and noncoding RNAs to regulate gene networks critical for normal physiology. Dysregulation of KDM3B has emerged as a contributing factor in the pathogenesis of multiple human diseases, such as cancer and neurodevelopmental disorders. In hematopoiesis, KDM3B functions as a regulator of gene expression programs essential for stem and progenitor cell maintenance. Loss-of-function mutations or deletions of KDM3B have been reported in myelodysplastic syndrome (MDS), acute myeloid leukemia (AML), and other hematologic malignancies. KDM3B deficiency leads to aberrant H3K9 methylation, resulting in transcriptional silencing of genes required for differentiation and genome stability. Overexpression of KDM3B has been observed in hepatocellular carcinoma and colorectal cancer, where it enhances glycolysis and angiogenesis through upregulation of HIF-1α target genes, thereby supporting tumor growth and survival.  KDM3B is implicated in neurodevelopmental and psychiatric disorders. Haploinsufficiency or mutations in the KDM3B gene have been associated with intellectual disability, developmental delay, and autism spectrum disorder. The underlying mechanisms are thought to involve disrupted chromatin states leading to impaired transcriptional programs governing neuronal differentiation, synapse formation, and plasticity. This product was carefully developed for performance and specificity using the following products from Horizon Discovery, a Revvity company: HAP1 KDM3B KO cells (Horizon Cat. No. HZGHC001145c010).
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human KDM3B
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
Structure: KDM3B is a 1761 amino acid protein with a predicted molecular weight of 191.6 kD.
Distribution: Nucleus
Function: Demethylate Lys-9 of histone H3 and play a central role in histone modification
Interaction: Lys-9 of histone H3
Biology Area: Chromatin Remodeling/Epigenetics
Molecular Family: Nuclear Markers
Antigen References: Kim JY, et al. 2012. Mol. Cell Biol. 32:2917-2933. Li S, et al. 2018. Cell Rep. 23:389-403. Diets IJ, et al. 2019. Am. J. Hum. Genet. 104:758-766. Xu X, et al. 2018. Leuk. Lymphoma. 59:204-213.
Gene ID: 51780
UniProt: View information about KDM3B on UniProt.org
Clone: W20097B
Regulatory Status: RUO
Other Names: Lysine-specific demethylase 3B, JmjCdomain-containing histone demethylation protein 2B, Jumonjidomain-containing protein 1B, Nuclear protein 5qNCA, [histoneH3]-dimethyl-L-lysine(9) demethylase 3B, C5orf7, JHDMB
Isotype: Rat IgG2a, κ