Biolegend, 648951, Purified anti-ATP5A1 Antibody, 25μg

ATP synthase F1 subunit alpha, also known as ATP5A1 or ATP5F1A, is a subunit of the mitochondrial complex V or F1F0 ATP synthase, which produces ATP from ADP. The enzyme ATP synthase has two complexes: F1, the soluble catalytic core, and Fo, which is composed of the proton channel. ATP5A1 is one of five different subunits that make up the main F1 core. Recent studies indicate ATP5A1 mutations lead to mitochondrial complex V deficiency which is a mitochondrial disorder with various symptoms such as dysmorphic features, psychomotor delays, hypotonia, growth impairment, cardiomyopathy, hepatomegaly, and hypoplastic renal structures. Furthermore, ATP5A1 is also associated with neurodevelopmental diseases.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human ATP5A1 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.03125 - 0.25 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: This antibody has been tested in knockout/knockdown systems for ICC. The antibody (clone W22077A) can cross-react with mouse in western blot (WB). For immunocytochemistry (ICC), we recommend fixation with 4% PFA followed by permeabilization with ice cold-methanol, or ice-cold methanol fixation. For immunohistochemistry on FFPE tissues (IHC-P), antigen retrieval with Citrate Buffer (Cat. No. 420901) or Tris-EDTA pH 9.0 (Cat. No. 422704) are recommended.
RRID: AB_3717127 (BioLegend Cat. No. 648951) AB_3717127 (BioLegend Cat. No. 648952)
Structure: ATP5A1 is a 553 amino acid protein with a predicted molecular weight of 60 kD.
Distribution: Mitochondrion inner membrane
Interaction: Interacts with ATPAF2, HRG, PLG, BLOC1S1, BCL2L1 isoform BCL-X(L), CLN5, PPT1, S100A1, and ABCB7
Cell Type: Neurons
Biology Area: Cell Biology, Mitochondrial Function, Neurodegeneration, Neuroscience
Molecular Family: Enzymes and Regulators, Mitochondrial Markers
Antigen References: Kataoka H, et al. 1991. Biochim Biophys Acta. 1089:393. Jonckheere A.I, et al. 2013. Brain. 136:1544. M.A L, et al. 2021. European Journal of Human Genetics.29:1719. Zech M, et al. 2022. Ann Neurol. 91:225.
Gene ID: 498
UniProt: View information about ATP5A1 on UniProt.org
Clone: W22077A
Regulatory Status: RUO
Other Names: ATP5F1A, alpha subunit ATP synthase isoform precursor (EC 3.6.1.34), ATP synthase alpha chain, mitochondrial, ATP synthase alpha subunit, ATP synthase alpha subunit precursor, ATP synthase F1 subunit alpha, ATP synthase subunit alpha
Isotype: Rat IgG2b, κ