Biolegend, 649706, Alexa Fluor® 488 anti-Pax-5 Antibody, 100μg

Pax5, also known as BSAP (B cell specific activator protein), is a member of the paired box (Pax) family of transcription factors. PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. Pax5 is the only member of the Pax family of transcription factors that is expressed in hematopoietic cells. During embryogenesis, Pax5 is transiently expressed in the brain of mice and in the mesencephalon and spinal cord of humans. Its expression is upregulated early in B cell development at the time of B cell commitment and is maintained throughout most subsequent stages. Suppression of Pax5 is essential for expression of Blimp-1 and the terminal differentiation of plasma cells. In the spleen, expression of Pax5 is higher in marginal zone B cells (B220+ CD21high CD23low) than in other B cells, especially the transition 1 stage (B220+ CD21- CD23-). In addition to its role in B cell development, Pax5 also affects VH-DJH heavy chain recombination as well as influencing the expression of many other B and non-B cell related proteins.  Its expression has also been detected in developing CNS and testis and so the encoded protein may also play a role in neural development and spermatogenesis. This gene is located at 9p13, which is involved in t(9;14)(p13;q32) translocations recurring in small lymphocytic lymphomas of the plasmacytoid subtype, and in derived large-cell lymphomas. This translocation brings the potent E-mu enhancer of the IgH gene into close proximity of the PAX5 promoter, suggesting that the deregulation of transcription of this gene contributes to the pathogenesis of these lymphomas. Alternatively spliced transcript variants encoding different isoforms have been described but their biological validity has not been determined.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant mouse Pax-5 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: The monoclonal 1H9 antibody recognizes both mouse and human Pax5. Additional reported applications (for the relevant formats) include: Western blotting1,2, immunohistochemical staining of formalin-fixed sections, and spatial biology (IBEX)4,5. NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).
Application References(PubMed link indicates BioLegend citation): Kallies A, et al. 2007. Immunity 26:555. (WB) McManus S, et al. 2011. EMBO J. 30:2388. (WB) Andricovich J, et al. 2016. J. Clin. Invest. 126:905. (ICFC) Pubmed Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Chen HY, et al. 2022. Elife. 11:. PubMed
RRID: AB_2562426 (BioLegend Cat. No. 649705) AB_2562427 (BioLegend Cat. No. 649706)
Structure: 391 amino acids with predicted molecular weight of 42 kD
Distribution: Nucleus
Function: Plays an important role in B-cell differentiation as well as neural development and spermatogenesis; involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene
Interaction: Interacts with DAXX; binds DNA as a monomer, binds TLE4
Cell Type: B cells
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Liao F, et al. 1992. J. Immunol. 148:2909. 2. Nutt SL, et al. 1997. Genes Dev. 11:476. 3. Nera KP, et al. 2006. Immunity 24:283. 4. Fuxa M, et al. 2007. Immunity 178:3031.
Gene ID: 5079
UniProt: View information about Pax-5 on UniProt.org
Clone: 1H9
Regulatory Status: RUO
Other Names: Paired box 5, B-cell lineage specific activator, BSAP, paired box homeotic gene 5
Isotype: Rat IgG2a, κ