Biolegend, 649952, Purified anti-CD3ε Antibody, 100μg

The CD3ε chain of the T-cell receptor (TCR)-CD3 complex is a type I membrane protein that plays a critical role in antigen recognition, TCR signal transduction and T-cell activation following binding to the peptide/MHC antigen complex. CD3ε is expressed by all mature T cells and forms a heteromeric complex with CD3 γ, δ and ζ chains, and either α/β or γ/δ chains of the TCR heterodimer. Upon activation, CD3 undergoes a conformational change, leading to exposure of the proline rich sequence in CD3ε and recruitment of Lck. CD3 is also important in T cell development, and deficiency of this gene has been associated with severe immunodeficiency.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of the intracellular domain of human CD3ε
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested WB, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704). For use in intracellular flow cytometry (ICFC), fixation/permeabilization with Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) is recommended. Permeabilization with True-Phos™ Perm Buffer is not recommended. This antibody (clone W23186F) is not recommended for use in immunocytochemistry (ICC) due to dim signal.
RRID: AB_3675183 (BioLegend Cat. No. 649951) AB_3675183 (BioLegend Cat. No. 649952)
Structure: CD3ε is a 207 amino acid protein with a predicted molecular weight of 23 kD.
Distribution: Plasma membrane
Function: Co-receptor
Interaction: CD3ε forms a heteromeric complex with CD3 γ, δ.and ζ chains, and either α/β or γ/δ chains of the TCR heterodimer.
Ligand/Receptor: CD3ε, as part of the TCR-CD3 complex, recognizes fragments of antigens bound to major histocompatibility complex molecules
Cell Type: NKT cells, T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules, TCRs
Antigen References: Schlossman S, et al. 1995. Leucocyte Typing V Oxford University Press New York. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego. Sedelies KA, et al. 2004. J Biol Chem. 279:26581-7. Rivollier A, et al. 2004. Blood. 104:4029-37. Scharschmidt E, et al. 2004. Mol. Cell Biol. 24:3860-73. Smeltz RB. 2007. J Immunol. 178:4786-92.
Gene ID: 12501
UniProt: View information about CD3epsilon on UniProt.org
Clone: W23186F
Regulatory Status: RUO
Other Names: T-cell surface glycoprotein CD3 epsilon chain, T3
Isotype: Rat IgG2a, κ