Biolegend, 650052, Purified anti-Claudin-6 Antibody, 100μg

Claudin-6 is a member of the claudin family of proteins, which consists of at least 24 members. Claudin proteins are integral membrane proteins characterized by 4 transmembrane regions and 2 extracellular loops that form tight junctions. Abnormal expression can disrupt tight junction integrity, playing an important role in the occurrence and development of cancers. Claudin-6 in particular, has been considered a therapeutic target because it is commonly expressed in tumor tissue but not in healthy adult tissue.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human Claudin-6
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P, ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 10 - 1 ug/mL is suggested. For immunocytochemistry, a concentration range of 10 - 1 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For use of this antibody in Immunocytochemistry (ICC), we recommend fixation/permeabilization using PFA-Triton X-100 and Methanol. We do not recommend PFA-Methanol fixation/permeabilization. For use of this antibody in immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P), we recommend antigen retrieval using Citrate Buffer, 10X (Cat. No. 420902), or Tris-EDTA buffer.
RRID: AB_3683486 (BioLegend Cat. No. 650051) AB_3683486 (BioLegend Cat. No. 650052)
Structure: Consists of four transmembrane domains and two extracellular loops. It is a 220 amino acid length protein with a predicted molecular weight of 23 kDa.
Distribution: Cellular membrane
Function: Tight junctions and cell adhesion
Antigen References: Reinhard K, et al. 2020. Science. 367(6476):446-453. Huan D, et al. 2021. Mol Med Rep. 24(3):677. Ushiku T, et al. 2012. Histophatology. 61(6):1043-56. Gowrikumar S, et al. 2019. Int J Mol Sci. 21:53.
Gene ID: 9074
UniProt: View information about Claudin-6 on UniProt.org
Clone: W22275B
Regulatory Status: RUO
Other Names: Claudin-6, Claudin 6, CLDN6, Skullin
Isotype: Rat IgG2a, λ