Biolegend, 650152, Purified anti-human CD20 Antibody, 100μg

CD20 is a cell surface protein primarily expressed on B lymphocytes which plays a key role in the regulation of B cell activation, proliferation, and differentiation. Encoded by the gene MS4A1, homo-oligomeric complexes of CD20 form calcium conductive ion channels that modulate signaling pathways necessary for B cell survival and immune responses. CD20 plays a role in B cell activation of downstream Src family kinases such as Lyn, Lck, and Fyn. Because of its selective expression on B cells CD20 has become a target for therapeutic monoclonal antibodies to treat conditions like B cell lymphomas and autoimmune diseases.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of the N terminus of human CD20 (cytosolic)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2˚C and 8˚C.
Application: IHC-P - Quality tested ICC, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704). For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with either of the following: -Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100 -Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol -100% ice-cold methanol only For use in intracellular flow cytometry (ICFC), it is recommended to fix/permeabilize with Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) or True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401). This product is not recommended for use in Western Blot.
RRID: AB_3699076 (BioLegend Cat. No. 650151) AB_3699076 (BioLegend Cat. No. 650152)
Structure: CD20 is a 297 amino acid protein with a predicted molecular weight of 33.1 kD.
Distribution: Cell membrane, B cells
Function: Calcium channel
Interaction: MHC class I and II, CD53, CD81, CD82
Cell Type: B cells, T cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: Morsy DE, et al. 2013. J Immunol. 191:3112. Lund FE and Randall TD. 2010. Nat Rev Immunol. 10:236. Beers SA, et al. 2010. Blood. 115:5191. Kuijpers TW, et al. 2010. J Clin Invest. 120:214.
Gene ID: 931
UniProt: View information about CD20 on UniProt.org
Clone: W23056F
Regulatory Status: RUO
Other Names: Cluster of differentiation 20 (CD20), membrane-spanning 4-domains subfamily A member 1 (MS4A1), B1, Bp35
Isotype: Rat IgG2a, κ