Biolegend, 650801, Purified anti-Histone H3 Phospho (Ser10) Antibody, 25μg

Histone H3 is phosphorylated at serine 10 during mitosis and is found to be involved in transcriptional activation, chromatin decondensation, and chromosome compaction during cell division, by the action of Aurora kinase and NIMA kinases.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Modified synthetic peptide conjugated to KLH
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation: The antibody was purified by antigen-affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality tested IF - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1 µg per ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The histone H3 pS10 antibody recognizes phosphorylation of human H3 protein at Ser10 residue and has been shown to be useful for Western blotting.
Product Citations: Friedman J, et al. 2018. J Immunother Cancer. 6:59. PubMed Han G, et al. 2018. Nat Protoc. 2.014583333. PubMed
RRID: AB_10896911 (BioLegend Cat. No. 650801) AB_10900065 (BioLegend Cat. No. 650802)
Structure: H3 is part of the nucleosome, comprised of an octameric complex with H2A, H2B, and H4 proteins.
Distribution: Nucleus
Function: H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin. Histones, therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication, and chromosomal stability.
Interaction: Two molecules of H3 form a heterotetramer with two molecules of H4.
Biology Area: Cell Biology, Chromatin Remodeling/Epigenetics, DNA Repair/Replication, Transcription Factors
Molecular Family: Phospho-Proteins
Antigen References: 1. Choi HS, et al. 2005. J. Biol. Chem. 280:13545. 2. Goto H, et al. 2002. Genes Cells 7:11. 3. Garcia BA, et al. 2005. Biochemistry 44:13202. 4. Hans F, et al. 2001. Oncogene 20:3021.
Regulation: H3 is regulated by acetylation, methylation, citrullination, phosphorylation, and ubiquitination.
Gene ID: 8290
UniProt: View information about Histone on UniProt.org
Clone: 11D8
Regulatory Status: RUO
Other Names: Histone-H3, H3
Isotype: Mouse IgG2b, κ