Biolegend, 650954, Ultra-LEAF™ Purified anti-human CXCL1 (GRO-α) Antibody, 1mg

The CXCL1 gene was initially identified by its constitutive overexpression in spontaneously transformed Chinese hamster fibroblasts. Originally purified as an autocrine growth factor from supernatants of a human melanoma cell line, CXCL1 is frequently overexpressed in many cancers, such as breast cancer, ovarian cancer, colorectal cancer, prostate cancer, and squamous cell carcinoma. CXCL1 is an ELR CXC chemokine, and it is structurally and functionally related to GRO2 (CXCL2), GRO3 (CXCL3), and interleukin-8 (CXCL8).  CXCL1, CXCL3, and IL-8 bind to the CXCR2 receptor with CXCL1 (GROα) having the highest affinity. CXCL1 is crucial for the recruitment of neutrophils to inflammatory sites. In addition, CXCL1 has pleiotropic effects on cell proliferation, tumor angiogenesis, invasion, and metastasis. Thrombin enhances the secretion of CXCL1 from tumor cells, and these two molecules together induce vascular regulatory proteins and growth factors involved in angiogenesis. This parallels activity of VEGF, Ang-2, CD31, KDR, CXCR2, MMP1, and MMP2 in HUVEC cells. In addition, PGE2 stimulates expression of CXCL1 in human colorectal carcinoma cells through activation of an EGFR-MAPK. Furthermore, CXCL1 (secreted by CRC cells) induces endothelial cell migration and tube formation in vitro and in vivo. CXCL1 is induced by inflammatory cytokines such as IL-1 and TNF. In addition, IL-17A enhances expression of CXCL1 by prolonging the half-life of its constitutively unstable mRNA.
1mg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Human CXCL1 (GRO-α)
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/μg of the protein (less than 0.001 ng/μg of the protein) as determined by the LAL test
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Neut - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by neutralizing the chemotaxis of mouse Baf3- mCXCR2 cells induced by recombinant human CXCL1 (GRO-α) (Cat. No. 574402) at 3 ng/mL. ND50 range: 0.09 - 0.7 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_3699077 (BioLegend Cat. No. 650953) AB_3699077 (BioLegend Cat. No. 650954)
Structure: Chemokine
Distribution: CXCL1 is produced by neutrophils, activated monocytes, fibroblasts, epithelial cells, and endothelial cells.
Function: CXCL1 chemoattracts neutrophils and induces cell proliferation, tumor angiogenesis, invasion, and metastasis. CXCL1 is induced by IL-1, TNF, thrombin, and PGE2.
Interaction: Neutrophils and endothelial cells
Ligand/Receptor: CXCR2
Biology Area: Cell Biology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: Anisowicz A, et al. 1987. P Natl Acad Sci USA. 84:7188. Caunt M, et al. 2006. Cancer Res. 66:4125-32. Wang D, et al. 2006. J Exp Med. 203:941-51. Datta S, et al. 2010. J Immunol. 184:1484-91.
Gene ID: 2919
UniProt: View information about CXCL1 on UniProt.org
Clone: A15050D
Regulatory Status: RUO
Other Names: FSP, Grow regulated oncogen 1 (GRO1), Grow regulated oncogen a (GRO a), melanoma growth stimulating activity (MGSA), MGSA-a, neutrophil activating protein 3 (NAP-3), SCYB1
Isotype: Mouse IgG2a, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.