Biolegend, 651652, Purified anti-Argonaute 3 Antibody, 100μg

Argonaute 3 (AGO3) is part of the Argonaute protein family, which plays an important role in RNA silencing and gene regulation. In the RNA interference (RNAi) pathway, AGO3 interacts with miRNAs, helping regulate gene expression post-transcriptionally by binding to specific RNA targets and blocking their translation into proteins. Although AGO3, like AGO1 and AGO4, lacks significant catalytic activity, it still plays a role in RNA binding and miRNA stabilization, allowing it to assist in gene silencing. This process is crucial in a variety of biological functions, including cell differentiation, immune responses, and development. Mutations or disruptions in AGO3 can have deleterious effects on reproductive health. AGO3 deficiency in Drosophila melanogaster (fruit flies) leads to a buildup of transposons in the germline and, consequently, sterility. This highlights AGO3's importance in maintaining reproductive viability by safeguarding genetic material.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human Argonaute 3
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: It is not recommended using this product in immunocytochemistry (ICC) or immunohistochemistry with FFPE tissue (IHC-P).
RRID: AB_3683488 (BioLegend Cat. No. 651651) AB_3683488 (BioLegend Cat. No. 651652)
Structure: Argonaute 3 is an 860 amino acid length protein with a predicted molecular weight of 97.3 kD and contains PAZ, Piwi, and guide RNA binding domains.
Distribution: Cytoplasmic
Function: Required for RNA-mediated gene silencing (RNAi)
Interaction: Interacts with EIF4B, IMP8, PRMT5 and TNRC6B
Biology Area: Stem Cells
Molecular Family: Enzymes and Regulators
Antigen References: Kirino Y, et al. 2009. Nat Cell Biol. 11:652-8. Nagao A, et al, 2010. RNA. 16:2503-15. Zhang Z, et al. 2016. Nat Plants. 2:16049.
Gene ID: 192669
UniProt: View information about Argonaute 3 on UniProt.org
Clone: W21012A
Regulatory Status: RUO
Other Names: Argonaute-3, Argonaute RISC Catalytic Component 3, Eukaryotic Translation Initiation Factor 2C, 3, EIF2C3, HAGO3
Isotype: Rat IgG2a, κ