Biolegend, 651751, Purified anti-SHP-2 Phospho (Tyr542) Antibody, 25μg

SHP-2 is encoded by the PTPN11 gene, is a well-known oncogenic member of the protein tyrosine phosphatase (PTP) family. SHP-2 regulates cellular functions by dephosphorylating targets downstream of PD1, EGFRvIII, HGF receptor, and cytokine receptors. Cellular processes controlled by SHP-2 include mitotic cycle, cell growth, differentiation, and oncogenesis. Phosphorylation of tyrosine 542 and 580 of SHP-2 in response to growth factor receptor activation leads to a stimulation in its tyrosine phosphatase activity. SHP-2 has been recognized as a potential therapeutic target for cancers including breast cancer, lung cancer, leukemia, gastric cancer, and oral cancer.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide of human SHP-2 phosphorylated at Tyr542
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_3683489 (BioLegend Cat. No. 651751) AB_3683489 (BioLegend Cat. No. 651752)
Structure: SHP-2 is a 593 amino acid protein with a molecular mass of 68 kD.
Distribution: Cytoplasm, Nucleus
Function: SHP-2 regulates cell proliferation, apoptosis, invasion, and metastasis
Interaction: SHP-2 interacts with PD1, EGFR, HGF receptor, RET, FGFR2, FGFR3, PI3K/AKT, and TLR3 signaling pathways.
Biology Area: Adaptive Immunity, Cell Biology, Immuno-Oncology, Immunology, Signal Transduction
Molecular Family: Protein Kinases/Phosphatase
Antigen References: Hao C, et al. 2020. Oncogene. 39:7166-7180. Marie D, et al. 2008. Cell Signal. 20:453-9. Cheng Kui QU. 2000. Cell Research. 10:279-288. Bennett AM, et al. 1994. Proc Natl Acad Sci USA. 91:7335.
Gene ID: 5781
UniProt: View information about SHP-2 Phospho Tyr542 on UniProt.org
Clone: A22019A
Regulatory Status: RUO
Other Names: PTPN11, CFC, NS1, JMML, SHP2, BPTP3, PTP2C, METCDS, PTP-1D, SH-PTP2, SH-PTP3, Syp, Protein Tyrosine Phosphatase Non-Receptor Type 11, SH2 Domain-Containing Protein Tyrosine Phosphatase 2
Isotype: Mouse IgG1, κ