Biolegend, 652301, Purified anti-human CD304 Antibody, 25μg

The membrane isoform of CD304 is a receptor that regulates angiogenesis and organogenesis in response to growth factors of the VEGF family. CD304 forms a complex with VEGFR2/KDR, resulting in increased cell migration and chemotaxis. In the nervous system, CD304 complexed with plexin mediates Sema-3A signaling and may be involved in axon guidance.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: 293E expressed human CD304 recombinant protein, b isoform
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel (1 µg/ml). It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_11203372 (BioLegend Cat. No. 652302)
Structure: Single-pass type I membrane protein, 103 kD. Contains two CUB motifs, domains similar to coagulation factors V and VIII, and a MAM domain in the extracellular domain. Isoform b is a soluble form.
Distribution: Membrane, Secreted (isoform b).
Function: CD304 is a VEGF co-receptor involved in axon guidance, angiogenesis. and development of cardiovascular system. The secreted isoform inhibits binding of VEGF-165 to cells.
Ligand/Receptor: CD304 is a receptor for members of VEGF family and pro-angiogenesis cytokines, such as VEGF-A165, VEGF-B, VEGF-E, and PIGF-2.
Biology Area: Angiogenesis, Cell Biology, Immunology, Signal Transduction
Molecular Family: CD Molecules
Antigen References: 1. Soker S, et al. 1998. Cell 92:735. 2. Shintani Y, et al. 2006. EMBO J. 25:3045. 3. He Z, et al. 1997. Cell 90:739. 4. Gagnon ML, et al. 2000. P. Natl. Acad. Sci. USA 97:2573. 5. Glinka Y, et al. 2011. Carcinogenesis 32:613.
Gene ID: 8829
UniProt: View information about CD304 on UniProt.org
Clone: 14H4
Regulatory Status: RUO
Other Names: Neuropilin 1
Isotype: Mouse IgG2b, κ