Biolegend, 652408, Alexa Fluor® 647 anti-mouse Ki-67 Antibody, 100μg

The nuclear protein Ki-67 was first identified by the monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the L428 Hodgkin lymphoma cell line. Ki-67 protein plays an essential role in ribosomal RNA transcription and cell proliferation. Expression of Ki-67 occurs during G1, S, G2, and M phase, while in G0 phase the Ki-67 protein is not detectable. Ki-67 is strongly expressed in proliferating cells and has been reported as a prognostic marker in various tumors.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: E. coli expressed partial mouse Ki-67 recombinant protein, 1816-2163 aa.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by our Ki-67 staining protocol below. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Medina-Reyes EI, et al. 2015. Environ Res. 136:424. PubMed Guillaumond F, et al. 2015. PNAS. 112:2473. PubMed Sharma SK, et al. 2015. J Immunol. 194:5529. PubMed Rodero MP, et al. 2014. J. Invest. Dermatol. 7:1991-7. PubMed
Product Citations: Sugitani N, et al. 2022. Cell Rep. 40:111371. PubMed Kuczynski EA, et al. 2022. EMBO Mol Med. 14:e15816. PubMed McCoy S 2014. Am J Physiol Lung Cell Mol Physiol. 307:618. PubMed Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed Garg G et al. 2019. Cell reports. 26(7):1854-1868 . PubMed Benet Z, et al. 2021. Cell Reports. 34(6):108733. PubMed Lu P, et al. 2021. Blood Cancer J. 11:39. PubMed Hu Y, et al. 2022. J Nanobiotechnology. 20:417. PubMed Chierico L, et al. 2017. PLoS One. 12(2):e0171815. PubMed Yang T, et al. 2022. Nat Commun. 13:4464. PubMed Tedeschi A, et al. 2020. Nat Commun. 11:1746. PubMed Shaikh H, et al. 2021. Front Immunol. 12:689896. PubMed Weis V, et al. 2016. Cell Mol Gastroenterol Hepatol. 2:131-157. PubMed Shimizu H, et al. 2019. JCI Insight. 4:4. PubMed Subbarayal B, et al. 2016. J Immunol. 197: 3464 - 3470. PubMed Zhang X, et al. 2020. Glia. . PubMed
RRID: AB_2562138 (BioLegend Cat. No. 652407) AB_2562139 (BioLegend Cat. No. 652408)
Structure: 325 kD protein containing a forkhead-associated domain (FHA) and 13 tandem repeats
Distribution: Nucleus and chromosome
Function: Required for cell cycle progression and proliferation
Interaction: Interacts with KIF15; binds to MKI67IP through FHA domain
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Starborg M, et al. 1996. J. Cell. Sci. 109:143. 2. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987. 3. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174. 4. Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750. 5. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295. 6. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.
Gene ID: 17345
UniProt: View information about Ki-67 on UniProt.org
Clone: 16A8
Regulatory Status: RUO
Other Names: KIA, proliferation-related Ki-67 antigen
Isotype: Rat IgG2a, κ