Biolegend, 652951, Purified anti-Beta-Arrestin 1 Antibody, 25μg

Beta-arrestin 1, a member of the arrestin family, is a crucial regulatory protein involved in G protein-coupled receptor (GPCR) signaling, modulating GPCR activity following receptor activation. Arrestins bind directly to phosphorylated GPCRs and preclude their interaction with other proteins, thus inhibiting further signaling and preventing overstimulation. Furthermore, binding of arrestins like Beta-arrestin 1 can result in receptor internalization, leading potentially to ligand-receptor decoupling and receptor recycling, or to receptor degradation and a reduction in the available receptors in the cell. Thus Beta-arrestin 1 regulates a wide variety of physiological processes including inflammation, metabolism, and cell migration, highlighting its importance in cellular communication and homeostasis.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant fragment of human Beta-Arrestin 1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 0.25 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody does not cross react with mouse Beta-Arrestin 1 in Western Blot. This product is not recommended for use in immunocytochemistry (ICC) or immunohistochemistry in formalin-fixed, paraffin-embedded tissues (IHC-P).
RRID: AB_3683490 (BioLegend Cat. No. 652951) AB_3683490 (BioLegend Cat. No. 652952)
Structure: Beta-arrestin 1 is a 418 amino acid protein with a predicted molecular weight of 47.1 kD.
Distribution: Cytoplasm, plasma membrane, and in clathrin-coated pits upon receptor internalization
Function: GPCR regulation
Interaction: Forms dimers and tetramers. Binds to phosphorylated GPCRs such as ADRB2, rhodopsin and CHRM2.
Cell Type: Neurons
Biology Area: Cardiovascular Biology, Cell Biology, Neuroscience, Signal Transduction
Molecular Family: Adaptor Proteins
Antigen References: Wess J, et al. 2023. Pharmacol Rev. 75:854-884. Gurevich VV and Gurevich EV. 2023. Curr Protoc. 3:e821. Latorraca NR, et al. 2021. Cell. 183:1813-1825.
Gene ID: 408
UniProt: View information about Beta-Arrestin 1 on UniProt.org
Clone: W20187A
Regulatory Status: RUO
Other Names: Beta-Arrestin-1 (ARBB1), Arrestin beta-1, Non-visual arrestin-2
Isotype: Rat IgG2b, κ