Biolegend, 653002, Purified anti-NF-κB p65 Antibody, 100μg

NF-κB p65 is a member of REL-like domain containing protein family, which forms a NF-κB complex with the other family members: NF-κB1 (p105/p50) or NF-κB2 (p100/p52). The NF-κB complex is inactivated and held in the cytoplasm by the NF-κB inhibitor IκB. In response to activation stimuli, IκB kinases (IKKs) phosphorylates IκB, resulting in degradation of IκB and liberation of NF-κB complex. The activated NF-κB complex translocates to the nucleus and binds to κB sites in the DNA of their target genes.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human NF-κB p65 recombinant protein (451-551 aa)
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality tested IP, KO/KD-WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1-1.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 2-10 μg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
Product Citations: De Maeyer RPH, et al. 2020. Nat Immunol. 21:615. PubMed
RRID: AB_2561612 (BioLegend Cat. No. 653001) AB_2561613 (BioLegend Cat. No. 653002)
Structure: 65 kD protein containing a Rel homology domain (RHD), an activation domain, and a 9aaTAD domain.
Distribution: The inactivated NF-κB complex containing p65 subunit is bound to IκB and is localized to cytoplasm. Upon activation, I-κB is phosphorylated and degraded. The activated NF-κB complex is in turn translocated to the nucleus as a transcription factor.
Function: NF-κB is a homodimeric or heterodimeric complex formed by the Rel-like domain-containing proteins. The most abundant form is p65 (RelA) - p50 (NF-κB1) heterodimer complex. The NF-κB complex is a ubiquitously expressed transcription factor which is involved in various biological functions, such as cell growth, tumorigenesis, differentiation, apoptosis, inflammation, and immune responses.
Interaction: Interacts with NF-κB1 (p105/p50) or NF-κB2 (p100/p52) to form heterodimeric NF-κB complex. Interacts with HDAC1, HDAC3, and CBP. Interaction with MEN1 inhibits transactivation activity of NF-κB complex.
Cell Type: B cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Li Z, et al. 1997. Mol. Cell. Biol. 17:6184. 2. Saccani S, et al. 2004. J. Exp. Med. 200:107. 3. Nolan GP, et al. 1991. Cell 64:961. 4. Chen LF, et al. 2001. Science 293:1653. 5. Hansen SK, et al. 1994. Mol. Cell. Biol. 14:2593. 6. Chapman NR, et al. 2002. Biochem J. 366:459.
Gene ID: 4790
UniProt: View information about NF-kappaB p65 on UniProt.org
Clone: 14G10A21
Regulatory Status: RUO
Other Names: Nuclear factor NF-kappa-B p65 subunit (NF-κB p65), Rel-A, transcription factor p65, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 (NFKB3)
Isotype: Mouse IgG2b, κ