Biolegend, 653005, APC anti-NF-κB p65 Antibody, 25tests

NF-κB p65 is a member of REL-like domain containing protein family, which forms a NF-κB complex with the other family members: NF-κB1 (p105/p50) or NF-κB2 (p100/p52). The NF-κB complex is inactivated and held in the cytoplasm by the NF-κB inhibitor IκB. In response to activation stimuli, IκB kinases (IKKs) phosphorylates IκB, resulting in degradation of IκB and liberation of NF-κB complex. The activated NF-κB complex translocates to the nucleus and binds to κB sites in the DNA of their target genes.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human NF-κB p65 recombinant protein (451-551 aa)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
RRID: AB_2563665 (BioLegend Cat. No. 653005) AB_2563666 (BioLegend Cat. No. 653006)
Structure: 65 kD protein containing a Rel homology domain (RHD), an activation domain, and a 9aaTAD domain.
Distribution: The inactivated NF-κB complex containing p65 subunit is bound to IκB and is localized to cytoplasm. Upon activation, I-κB is phosphorylated and degraded. The activated NF-κB complex is in turn translocated to the nucleus as a transcription factor.
Function: NF-κB is a homodimeric or heterodimeric complex formed by the Rel-like domain-containing proteins. The most abundant form is p65 (RelA) - p50 (NF-κB1) heterodimer complex. The NF-κB complex is a ubiquitously expressed transcription factor which is involved in various biological functions, such as cell growth, tumorigenesis, differentiation, apoptosis, inflammation, and immune responses.
Interaction: Interacts with NF-κB1 (p105/p50) or NF-κB2 (p100/p52) to form heterodimeric NF-κB complex. Interacts with HDAC1, HDAC3, and CBP. Interaction with MEN1 inhibits transactivation activity of NF-κB complex.
Cell Type: B cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Li Z, et al. 1997. Mol. Cell. Biol. 17:6184. 2. Saccani S, et al. 2004. J. Exp. Med. 200:107. 3. Nolan GP, et al. 1991. Cell 64:961. 4. Chen LF, et al. 2001. Science 293:1653. 5. Hansen SK, et al. 1994. Mol. Cell. Biol. 14:2593. 6. Chapman NR, et al. 2002. Biochem J. 366:459.
Gene ID: 4790
UniProt: View information about NF-kappaB p65 on UniProt.org
Clone: 14G10A21
Regulatory Status: RUO
Other Names: Nuclear factor NF-kappa-B p65 subunit (NF-κB p65), Rel-A, transcription factor p65, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 (NFKB3)
Isotype: Mouse IgG2b, κ