Biolegend, 653812, PerCP/Cyanine5.5 anti-GATA3 Antibody, 100tests

GATA3 functions as a major regulator of T helper 2 (Th2) cell differentiation in immune cells. GATA3 expression is upregulated through IL-4 receptor signaling or the Notch-mediated pathway, which results in production of IL-4, IL-5, and IL-13 cytokines, responsible for preferential differentiation of Th2-cells. GATA3 has been shown to regulate early developmental processes of T cells, such as T cell commitment, positive selection, and post-commitment CD4 + T cell survival. In the mammary gland, GATA3 plays an important role in differentiation and commitment of luminal epithelial cells. In a mouse model, exogenous expression of GATA3 in undifferentiated breast carcinoma cells induces differentiation and suppresses spreading of the tumor cells, suggesting that GATA3 is involved in preventing malignancy of breast cancer. GATA3 has been reported to be essential in the development of a variety of normal tissues. Defect in GATA3 results in hypoparathyroidism, sensorineural deafness, and renal dysplasia (HDR syndrome).
100tests
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human GATA3 recombinant protein (1-258 aa).
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Product Citations: Michela Miani et al. 2018. Cell metabolism. 28(4):557-572 . PubMed Alam A, et al. 2022. Cancer Cell. 40:153. PubMed Zhang M, et al. 2023. JCI Insight. 8: . PubMed Ye L, et al. 2022. Cell Metab. 34:595. PubMed Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed Richardson JR, et al. 2018. Front Immunol. 2.182638889. PubMed Liu Y, et al. 2021. Prostate. 81:29. PubMed Matisz CE, et al. 2018. Am J Pathol. 188:2589. PubMed Hayashi K, et al. 2020. Nat Commun. 4.832638889. PubMed
RRID: AB_2563218 (BioLegend Cat. No. 653811) AB_2563219 (BioLegend Cat. No. 653812)
Structure: 48 kD protein containing two GATA-type zinc finger domains responsible for DNA binding and two trans-activating domains at N-terminus.
Distribution: Nucleus
Function: GATA3, a transcriptional activator, binds to a consensus DNA sequence 5'-(A/T)GATA(A/G)-3' in the promoter region of genes, regulates T cell development, TH2 cell differentiation, and luminal cell proliferation.
Interaction: Interacts with Smad3, FOG1, FOG2, and PU.1.
Biology Area: Cell Biology, Chromatin Remodeling/Epigenetics, Immunology, Signal Transduction, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Yagi R, et al. 2011. Int. Immunol. 23:415. 2. Chou J, et al. 2010. J. Cell Physiol. 222:42. 3. Ho IC, et al. 2009. Nat. Rev. Immunol. 9:125. 4. Rothenberg EV, et al. 2008. Semin. Immunol. 20:236. 5. Kouros-Mehr H, et al. 2008. Curr. Opin. Cell Biol. 20:164. 6. Van Esch H, et al. 2000. Nature 406:419.
Gene ID: 262514462
UniProt: View information about GATA3 on UniProt.org
Clone: 16E10A23
Regulatory Status: RUO
Other Names: GATA-binding factor 3, trans-acting T-cell-specific transcription factor GATA-3
Isotype: Mouse IgG2b, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.