Biolegend, 654004, Alexa Fluor® 647 anti-IDO1 Antibody, 100μg

IDO1 is also known as Indolamine 2,3-dioxygenase, Indole 2,3-dioxygenase, and Indoleamine-pyrrole 2,3-dioxygenase. IDO is a ubiquitously expressed cytoplasmic protein with a predicted molecular weight of approximately 45 kD. Indoleamine 2,3-dioxygenase (IDO) is one the best known IFN-γ inducible genes. The product of IDO gene catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine. IDO has been implicated to protect against intracellular and extracellular pathogens. It also has been shown to maintain the special immune suppressive status of immune-privileged sites such as the brain, eyes, kidney, and placenta.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant mouse IDO1.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Clone 2E2/IDO1 reacts with mouse IDO1. It does not appear to react with mouse IDO2. Clone 2E2/IDO1 is the same clone as anti-IDO1 clone 2E2.6.
Product Citations: Michela Miani et al. 2018. Cell metabolism. 28(4):557-572 . PubMed Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed Chen C, et al. 2020. Front Cell Neurosci. 14:8. PubMed Molina MS, et al. 2021. Front Immunol. 12:699128. PubMed Li A, et al. 2018. Cancer Lett. 431:54. PubMed
RRID: AB_2564584 (BioLegend Cat. No. 654003) AB_2564585 (BioLegend Cat. No. 654004)
Structure: 403 amino acids with a predicted molecular weight of 45 kD.
Distribution: Cytoplasm
Function: Indoleamine 2,3-dioxygenase (IDO) is an IFN-γ inducible gene. It catalyzes the degradation of the essential amino acid L-tryptophan to N-formylkynurenine. IDO has been implicated to protect against intracellular and extracellular pathogens.
Interaction: This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin.
Biology Area: Cell Biology, Immunology
Antigen References: 1. Habara-Ohkubo A, et al. 1991. Gene 105:221. 2. Munn DH, et al. 2002. Science 297:1867. 3. Frumento G, et al. 2002. J. Exp. Med. 196:459. 4. Muller AJ, et al. 2005. Nature Med. 11:312. 5. Lob S, et al. 2009. Nature Rev. Cancer 9:445.
Gene ID: 15930
UniProt: View information about IDO1 on UniProt.org
Clone: 2E2/IDO1
Regulatory Status: RUO
Other Names: Indolamine 2,3-dioxygenase, Indole 2,3-dioxygenase, Indoleamine-pyrrole 2,3-dioxygenase
Isotype: Mouse IgG1, κ