Biolegend, 655602, Purified anti-Bin1 Antibody, 100μg

Bridging integrator 1 (Bin1) is a conserved member of the BAR family of genes that have been implicated in diverse cellular processes including endocytosis, cell polarity, programmed cell death, and stress signaling. It is involved in membrane remodeling and is one of the important genes in predicting risk for Alzheimer's disease (AD). Alternative splicing of the gene results in ten transcript variants. Isoforms 1-7 are expressed predominantly in the central nervous system. Isoform 8 is expressed specifically in muscle, and defects in BIN1 cause autosomal recessive myotubular myopathy. Isoforms 9 and 10 are ubiquitously expressed and may act as tumor repressors.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Fragment of human Bin1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IP, FC, ELISA - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 4.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Chapuis J, et al. 2013. Mol. Psychiatry doi: 10.1038/mp.2013.1.
RRID: AB_2562162 (BioLegend Cat. No. 655602)
Structure: Ten isoforms, 409-593 amino acids, predicted molecular weight of 45-70 kD
Distribution: Isoforms 1-7 predominantly cytosolic, isoforms 8-10 found in both the nucleus and/or cytosol
Function: Plays important roles in membrane remodeling, cell polarity, programmed cell death, and stress signaling
Interaction: Heterodimer with AMPH; binds SH3GLB1, SYNJ1, DNM1, clathrin and AP2A2; interacts with MYC, BIN2 and HCV NS5A.
Ligand/Receptor: SH3GLB1
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure, DNA Repair/Replication, Immunology, Signal Transduction, Transcription Factors
Antigen References: 1. Kingwell K. 2013. Nature Reviews Neurology. 9:184. 2. Chapuis J, et al. 2013. Mol. Psychiatry doi: 10.1038/mp.2013.1. 3. Schellenberg GD and Montine TJ. 2012. Acta. Neuropathol. 124:305. 4. Ganesan S. 2011. Sci. Signal. 4:15. 5. Cowling BS, et al. 2012. PLoS. Genet. 8:e1002595. 6. Lambert JC and Amouyel P. 2011. Curr. Opin. Genet. Dev. 21:295. 7. Romero NB. 2010. Neuromuscul. Disord. 20:223. 8. Prendergast GC, et al. 2009. Biochim. Biophys. Acta. 1795:25.
Gene ID: 274
UniProt: View information about Bin1 on UniProt.org
Clone: 99D
Regulatory Status: RUO
Other Names: Amphiphysin 2, AMPHL, SH3P9, Box-Dependent Myc-Interacting Protein 1, Myc Box-Dependent-Interacting Protein 1
Isotype: Mouse IgG2b, κ