Biolegend, 656805, PE anti-Nestin Antibody, 25tests

Nestin is a class VI intermediate filament protein originally found in neuronal stem cells, and is required for survival and renewal of neural progenitor cells. In mixtures, Nestin forms heterodimers with vimentin or internexin, participating in the remodeling of the cell. Nestin was found to be expressed on vascular endothelial progenitor cells and various neoplasms. It is proposed to be a novel angiogenesis marker and cancer therapeutic target.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.
Application References(PubMed link indicates BioLegend citation): Jozef S, et al. 2012. Biomed. Pap. Med. Fac. 156:135. (IHC)
Product Citations: Kuang YL, et al. 2019. Stem Cell Res. 37:101434. PubMed López-Valero I, et al. 2020. Theranostics. 10:5120. PubMed Gerace D, et al. 2021. Stem Cell Research. 53:102371. PubMed
RRID: AB_2566381 (BioLegend Cat. No. 656805) AB_2566382 (BioLegend Cat. No. 656806)
Structure: 1621 amino acids with predicted molecular weight of 200-220 kD
Distribution: Cytosol
Function: Required for survival and renewal of neural progenitor cells; plays roles in angiogenesis and tumor formation
Interaction: Vimentin, alpha-internexin and FHOD3
Cell Type: Neural Stem Cells
Biology Area: Angiogenesis, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family: Intermediate Filaments
Antigen References: 1. Matsuda Y, et al. 2013. World J. Gastroenterol. 19:42. 2. Matsuda Y, et al. 2012. Med. Mol. Morphol. 45:59. 3. Krupkova O Jr, et al. 2010. Neoplasma. 57:291. 4. Hombach-Klonisch S, et al. 2008. Arch. Immunol. Ther. Exp. (Warsz). 56:165. 5. Pilkington GJ, et al. 2005. Cell Prolif. 38:423.
Gene ID: 10763
UniProt: View information about Nestin on UniProt.org
Clone: 10C2
Regulatory Status: RUO
Other Names: NES
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.