Biolegend, 656952, Purified anti-TMEM97 Antibody, 100μg

Sigma intracellular receptor 2 (σ2R), also known as SIGMAR2 or TMEM97, is a transmembrane protein that is associated with the endoplasmic reticulum (ER), membranes and lysosomes. It is widely distributed in normal tissues such as the brain, lungs, heart, skeletal muscles, testis, and ovary. TMEM97 has been demonstrated to regulate the cholesterol transporter NPC1 in maintaining cholesterol homeostasis. Elevated expression of TMEM97 is a potential prognostic biomarker for breast, lung, colon, ovarian, bladder and oral squamous cancers. TMEM97 is also implicated in several neurological disorders and age-related diseases such as Alzheimer's disease.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human TMEM97 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: For use in immunohistochemistry on FFPE tissues (IHC-P), antigen retrieval with Citrate Buffer (Cat. No. 420901) is recommended. This antibody (clone W22180E) does not cross-react with mouse TMEM97 in Western Blot. We do not recommend this antibody for use in immunocytochemistry (ICC).
RRID: AB_3717126 (BioLegend Cat. No. 656951) AB_3717126 (BioLegend Cat. No. 656952)
Structure: TMEM97 is a 176 amino acid protein with a predicted molecular weight of 21 kD.
Distribution: Widely expressed in normal tissues with increased expression in proliferating cancer cells.
Function: Controlling cellular cholesterol levels, cancer biomarker, modulates the cytosolic Ca2+ concentration, dopaminergic transmission, and cocaine-induced addiction behavior.
Interaction: Interacts with NPC1, PGRMC1, LDLR, histatin 1/HTN1 and TSPO.
Cell Type: Microglia, Neurons
Biology Area: Cell Biology, Neurodegeneration, Neuroscience
Molecular Family: GPCR
Antigen References: Bartz, F. et al. 2009. Cell Metab. 10:63. Narayanan S, et al. 2011. Future Med Chem. 3:79. Oyer HM, et al. 2019. Front Pharmacol. 10:1141. Yi B, et al. 2017. J Neurochem. 140:561. Vazquez-Rosa E, et al. 2019. ACS Chem Neurosci.10:1595. Riad, A. et al. 2020. Mol Neurobiol. 57:3803.
Gene ID: 27346
UniProt: View information about TMEM97 on UniProt.org
Clone: W22180E
Regulatory Status: RUO
Other Names: MAC30, Meningioma-associated protein 30; σ2R; Sigma intracellular receptor 2, Sigma-2 receptor, Transmembrane protein 97, S2R
Isotype: Rat IgG2a, κ