Biolegend, 657404, Alexa Fluor® 488 anti-Tubulin Beta 3 (TUBB3) Antibody, 100μg

Tubulin is the main component of microtubules. In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation. Mutations in this gene cause congenital fibrosis of the type 3 extraocular muscles. Tubulin beta 3 (TUBB3) is also found in a wide range of tumors. Studies indicate that it is a predictive and prognostic marker in various tumors.
100μg
Verified Reactivity: Mouse, Rat, Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested IHC-F, 3D IHC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. For immunohistochemical staining on frozen tissue sections, the suggested use is 1.25 - 5.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.
Application References(PubMed link indicates BioLegend citation): Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Metzner K, et al. 2022. Front Cell Dev Biol. 10:965382. PubMed Yu C, et al. 2022. Invest Ophthalmol Vis Sci. 63:1. PubMed Gao X, et al. 2021. Nature. 589:591. PubMed Zhang J, et al. 2020. J Cell Mol Med. 24:4547. PubMed
RRID: AB_2562669 (BioLegend Cat. No. 657403) AB_2562670 (BioLegend Cat. No. 657404)
Structure: 450 amino acids with predicted molecular weight of 50 kD
Distribution: Cytosol
Function: Plays important roles in neuronal cell proliferation and differentiation
Interaction: Alpha tubulin, kinesin and dynein
Cell Type: Mature Neurons
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: Microtubules
Antigen References: 1. Katsetos CD, et al. 2003. J. Child Neurol. 18:851. 2. Mobarakeh ZT, et al. 2012. Cell Biol. Int. Rep. (2010) 19:e00015. 3. Locher H, et al. 2013. Differentiation. 85:173. 4. Karki R, et al. 2013. Expert Opin. Ther. Targets. 17:461. 5. Mariani M, et al. 2011. Curr. Mol. Med. 11:726. 6. Koh Y, et al. 2009. Ann. Oncol. 20:1414.
Gene ID: 10381
UniProt: View information about Tubulin beta-3 on UniProt.org
Clone: AA10
Regulatory Status: RUO
Other Names: β-3 Tubulin, Neuronal class III beta-tubulin, β3-tub
Isotype: Mouse IgG2a, κ