Biolegend, 657802, Purified anti-Lin28A Antibody, 100μg

LIN28A is a highly conserved RNA-binding protein and reprogramming factor that plays important roles in development, self-renewal of stem cells, glucose metabolism and tumorigenesis. Lin28A modulates not only miRNAs, particularly the let-7 family miRNAs, but also the translation of mRNAs that are important for cell growth and metabolism. LIN28A/B has been observed to be overexpressed in a wide range of solid tumors and hematological malignancies, and has emerged as a new therapeutic target candidate for cancer.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human Lin28A from E. coli
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.025 - 1.0 µg per ml (1:500 - 1:20,000 dilution). For immunocytochemistry, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For flow cytometric staining, the suggested use of this reagent is 0.06 - 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: HepG2 and HEL cells were selected for specificity testing because they express LIN28B mRNA but lack detectable LIN28A expression (source: Human Protein Atlas). This clone is specific for Lin28A does not recognize Lin28B.
RRID: AB_2562650 (BioLegend Cat. No. 657802)
Structure: 209 amino acids, predicted molecular weight of 28 kD with the characteristic of a cold shock domain (CSD) and two tandem Cys-Cys-His-Cys (CCHC)-type zinc-binding motifs (CCHCx2)
Distribution: Cytoplasm and nucleus; localizes to cytoplasmic processing bodies and stress granules
Function: Development, self-renewal of stem cells, glucose metabolism and tumorigenesis; regulates Let-7 family miRNA, translation of mRNA
Interaction: EIF3S2, NCL and ZCCHC11/TUT4
Cell Type: Embryonic Stem Cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics, Immunology, Protein Synthesis, Signal Transduction, Stem Cells
Antigen References: 1. Shyh-Chang N, et al. 2013. Cell Stem Cell. 12:395. 2. Thornton JE, et al. 2012. Trends Cell Biol. 22:474. 3. Huang Y. 2012. Wiley Interdiscip. Rev. RNA. 3:483. 4. Cho J, et al. 2012. Cell. 151:765. 5. Li N, et al. 2012. J. Biol. Chem. 287:17386. 6. Zhu H, et al. 2011. Cell 147:81.
Gene ID: 79727
UniProt: View information about Lin28A on UniProt.org
Clone: 14E6-4E6
Regulatory Status: RUO
Other Names: Protein Lin-28 Homolog A,Lin-28A, CSDD1,ZCCHC1, Zinc Finger CCHC Domain-Containing Protein 1
Isotype: Mouse IgG2a, κ