Biolegend, 657808, Alexa Fluor® 488 anti-Lin28A Antibody, 100tests

LIN28A is a highly conserved RNA-binding protein and reprogramming factor that plays important roles in development, self-renewal of stem cells, glucose metabolism and tumorigenesis. Lin28A modulates not only miRNAs, particularly the let-7 family miRNAs, but also the translation of mRNAs that are important for cell growth and metabolism. LIN28A/B has been observed to be overexpressed in a wide range of solid tumors and hematological malignancies, and has emerged as a new therapeutic target candidate for cancer.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human Lin28A from E. coli
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: This clone is specific for Lin28A does not recognize Lin28B.
RRID: AB_2924623 (BioLegend Cat. No. 657807) AB_2924623 (BioLegend Cat. No. 657808)
Structure: 209 amino acids, predicted molecular weight of 28 kD with the characteristic of a cold shock domain (CSD) and two tandem Cys-Cys-His-Cys (CCHC)-type zinc-binding motifs (CCHCx2)
Distribution: Cytoplasm and nucleus; localizes to cytoplasmic processing bodies and stress granules
Function: Development, self-renewal of stem cells, glucose metabolism and tumorigenesis; regulates Let-7 family miRNA, translation of mRNA
Interaction: EIF3S2, NCL and ZCCHC11/TUT4
Cell Type: Embryonic Stem Cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics, Immunology, Protein Synthesis, Signal Transduction, Stem Cells
Antigen References: Shyh-Chang N, et al. 2013. Cell Stem Cell. 12:395-406. Thornton JE, et al. 2012. Trends Cell Biol. 22:474-82. Huang Y. 2012. Wiley Interdiscip Rev RNA. 3:483-94. Cho J, et al. 2012. Cell. 151:765-777. Li N, et al. 2012. J Biol Chem. 287:17386-17397. Zhu H, et al. 2011. Cell 147:81-94.
Gene ID: 79727
UniProt: View information about Lin28A on UniProt.org
Clone: 14E6-4E6
Regulatory Status: RUO
Other Names: Protein Lin-28 Homolog A, Lin-28A, CSDD1, ZCCHC1, Zinc Finger CCHC Domain-Containing Protein 1
Isotype: Mouse IgG2a, κ