Biolegend, 658402, Purified anti-NFATc2 Antibody, 100μg

NFATc2 is a family member of the NFAT (calcineurin-dependent nuclear factor of activated T cells) transcription factors, regulating expression of the genes involved in T-cell activation and proliferation. In resting T cells, NFATc2 protein is phosphorylated and is present in the cytoplasm. Upon stimulation, NFATc2 protein is dephosphorylated by calcium dependent phosphatase, resulting in the translocation of NFATc2 protein to the nucleus, where it induces expression of target genes. In addition to its roles in immune cells, NFATc2 is revealed to mediate VEGF signaling, transactivating the gene encoding cyclooxygenase 2 (COX2). The production of prostaglandins by cycloxoygenase 2 is required for the migration of endothelial cells and angiogenesis. NFATc2 is also suggested to participate in tumorigenesis and invasive migration of tumor cells.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: N-terminal Peptide-KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2.0 - 4.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2562881 (BioLegend Cat. No. 658402)
Structure: 925 amino acids, predicted molecular weight of 100 kD; contains two transactivation domains and a Rel homology domain (RHD) responsible for DNA binding
Distribution: Phosphorylated form in cytoplasm; translocates to the nucleus when dephosphorylated
Function: Transcription factor, regulating T cell development, differentiation, and activation
Interaction: Forms complex with other family members; interacts with p300/CBP, MAF, GATA4, and NFATC2IP
Cell Type: Mesenchymal Stem Cells, Embryonic Stem Cells, B cells, Neural Stem Cells
Biology Area: Angiogenesis, Cell Biology, Cell Cycle/DNA Replication, Immunology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Gerlach K, et al. 2012. Cancer Res. 72:4340. 2. Ohoka Y, et al. 2011. J. Immunol. 186:733. 3. Nguyen TN, et al. 2010. Mol. Immunol. 47:2314. 4. Alev C, et al. 2009. Cell Cycle. 8:1803. 5. Becker M, et al. 2009. J. Immunol. 182:6136. 6. Horsley V, et al. 2002. J. Cell Biol. 156:771.
Gene ID: 4773
UniProt: View information about NFATc2 on UniProt.org
Clone: 16A12A31
Regulatory Status: RUO
Other Names: Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2 (NFATc2), T-cell transcription factor NFAT1, NFAT pre-existing subunit (NFATp)
Isotype: Mouse IgG1, κ