Biolegend, 659152, Purified anti-EHF Antibody, 100μg

ETS homologous factor (EHF), also known as epithelium-specific ETS transcription factor 3 (ESE-3), is a member of the ETS family of transcription factors expressed in various epithelial cell types, which participates in the oncogenesis of many malignant tumors through regulating the cell proliferation and differentiation. The genome-wide association study (GWAS) identified that genetic markers of lung disease severity in cystic fibrosis (CF) led to renewed interest in the potential importance of EHF in lung disease. Studies have revealed that EHF transcript variants such as EHF-LF and EHF-SF may have different impacts on epithelial cell differentiation and can promote or inhibit the epithelial mesenchymal transition (EMT) phenomenon. EHF also has been found in kidney, lung and somatotroph tumors and may serve as a prognostic biomarker. According to recent studies, EHF has been shown to inhibit pancreatic ductal adenocarcinoma (PDAC) metastasis through up-regulation of E-cadherin.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human EHF protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: For immunocytochemistry (ICC), we recommend 4% PFA fixation followed by permeabilization with Triton X-100 or ice-cold methanol. This antibody (clone W22125A) is not suitable for IHC. This antibody (clone W22125A) does not cross-react with mouse in western blot (WB).
RRID: AB_3717146 (BioLegend Cat. No. 659151) AB_3717146 (BioLegend Cat. No. 659152)
Structure: EHF is a 300 amino acid protein with a predicted molecular weight of 35 kD.
Distribution: Nucleus
Function: Transcriptional activator, regulating epithelial cell differentiation
Biology Area: Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability, Transcription Factors
Molecular Family: Tumor Suppressors
Antigen References: Fossum S.L, et al. 2017. J Biol Chem. 292:10938. Wright F. A, et al. 2011. Nat Genet. 43:539. Sakamoto K, et al. 2021. Oncogenesis. 10:26. Liu J, et al. 2019. J Exp Med. 216:656.
Gene ID: 26298
UniProt: View information about EHF on UniProt.org
Clone: W22125A
Regulatory Status: RUO
Other Names: EHF; Epithelium-specific ETS transcription factor 3; ESE-3; ESE3 transcription factor; ETS domain-containing transcription factor; ETS homologous factor.
Isotype: Rat IgG2a, κ