Biolegend, 659607, Alexa Fluor® 488 anti-human IL-31 Antibody, 25tests

IL-31, produced mainly by activated CD4 + T cells, is a newly discovered helical cytokine belonging to the gp130/IL6 cytokine family. IL-31 is closely related in structure to IL-6, IL-11, and IL-27. Binding of IL-31 to its receptor activates JAK/STAT, PI3K/AKT, and MAPK pathways. IL-31 acts on a broad range of immune and non-immune cells and thus possesses potential pleiotropic physiological functions, such as regulating hematopoiesis and immune response. Human IL-31 is also involved in inflammatory bowel disease, airway hypersensitivity, and dermatitis.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full length human IL-31 recombinant protein expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Product Citations: Bai Z, et al. 2024. Nature. 702:634. PubMed
RRID: AB_2616660 (BioLegend Cat. No. 659607) AB_2616661 (BioLegend Cat. No. 659608)
Structure: 161 aa with with a putative 23 aa signal peptide that is cleaved to generate a 138 aa mature protein.
Distribution: Secreted by activated CD4 T cells.
Function: IL-31 belongs to the IL-6 family of cytokines and plays a role in skin inflammation.
Ligand/Receptor: Binds to IL-31 receptor complex that consists of two receptor subunits, IL31RA and OSMR.
Cell Type: Eosinophils, T cells
Biology Area: Cell Biology, Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. McCandless EE, et al. 2014. Vet. Immunol. Immunopathol. 157:42. 2. Arai I, et al. 2013. Exp. Dermatol. 22:669. 3. Dai X, et al. 2013. PLoS One. 8:e67666. 4. Stott B, et al. 2013. J. Allergy Clin. Immunol. 132:446. 5. Hartmann K, et al. 2013. J. Allergy Clin. Immunol. 132:232. 6. Baron JM and Lüscher B. 2012. Acta. Derm. Venereol. 92:5.
Gene ID: 386653
UniProt: View information about IL-31 on UniProt.org
Clone: 1D10B31
Regulatory Status: RUO
Other Names: Interleukin-31
Isotype: Mouse IgG2b, κ