Biolegend, 659802, Purified anti-OCT2 Antibody, 100μg

OCT2 is a member of the POU family of transcription factors that was found to be expressed predominantly in B lymphocytes, activated T lymphocytes, and neuronal cells. OCT2 binds to the conserved octamer DNA element, ATGCAAAT, which was discovered in the promoters and enhancers of immunoglobulin (Ig) genes. In addition to regulating Ig gene expression, OCT2 controls several B cell specific genes required for proliferation and differentiation, such as CD20, CD36, and CD79a. OCT2 was found to have different isoforms produced by alternative splicing. The OCT2 isoforms exert distinct functional activities and can act as transcriptional activators or repressors.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human OCT2 recombinant protein (1-200 aa)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone may produce a non-specific band of ~75 kD by western blot.
RRID: AB_2563348 (BioLegend Cat. No. 659801) AB_2563350 (BioLegend Cat. No. 659802)
Structure: 479 amino acids, predicted molecular weight of 51 kD (isoform 1). Contains a POU-specific domain and a Homeobox DNA-binding domain.
Distribution: Expressed predominantly in B cells, activated T cells, and neurons.
Function: OCT2 is a transcription factor, regulating the transactivation of B cell-specific genes involved in immunoglobulin production, B cell differentiation, and proliferation.
Interaction: Interacts with OBF-1, NR3C1, AR and PGR.
Cell Type: B cells, Neurons, T cells
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Heckman CA, et al. 2006. Oncogene. 25:888. 2. García-Cosío M, et al. 2004. Mod. Pathol. 17:1531. 3. Corcoran LM, et al. 2001. J. Immunol. 172:2962. 4. Shore P, et al. 2002. Nucleic Acids Res. 30:1767. 5. Corcoran L, et al. 2002. Int. Immunol. 14:1099. 6. Latchman DS. 1996. Int. J. Biochem. Cell Biol. 28:1081. 7. Kemler I, et al. 1990. FASEB J. 4:1444.
Gene ID: 5452
UniProt: View information about OCT2 on UniProt.org
Clone: 2B10B19
Regulatory Status: RUO
Other Names: Octamer-binding protein 2, Octamer-binding transcription factor 2 (OTF-2), POU domain, class 2, transcription factor 2 (POU2F2)
Isotype: Mouse IgG1, κ