Biolegend, 660203, Direct-Blot™ HRP anti-STAT5 Antibody, 100microl

STAT5 is a member of the signal transducer and activator of transcription factors (STAT) family, mediating growth and cytokine signaling. STAT5 consists of two closely related family members, STAT5A and STAT5B, which exhibit 96% sequence homology and are functionally redundant.  Upon activation, STAT5 is phosphorylated by receptor tyrosine kinases and, in turn, forms homodimers or hererodimers with other family members through its SH2 domains. The dimerized STAT5 translocates to the nucleus and binds to the STAT5 response element (TTCXXXGAA). STAT5 plays crucial regulatory roles in cell proliferation, anti-apoptosis, early B cell differentiation, and T cell development.
100microl
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial human STAT5A recombinant protein (627-794 a.a.) expressed in E. coli.
Formulation: This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation: The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:4000. The optimal dilution should be determined by titration for each individual assay of interest. 25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.
Application Notes: This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
RRID: AB_2616893 (BioLegend Cat. No. 660203)
Structure: 794 amino acids, predicted molecular weight of 91 kD; contains a SH2 domains responsible for homodimerization or heterodimerization.
Distribution: Cytoplasm; phosphorylated form translocates to the nucleus.
Function: STAT5 is a transcription regulator and is involved in cell differentiation, proliferation, and survival, in response to several cytokines, such as interleukins, erythropoietin, growth hormone, thrombopoietin, and prolactin.
Interaction: Forms a homodimer or a heterodimer with other family members. Interacts with NCOA1, SOCS7, CBP, p300, SMRT and ERBB4.
Cell Type: B cells, Tregs
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Signal Transduction, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Bell BD, et al. 2013. Nat. Immunol. 14:364. 2. Cholez E, et al. 2012. Leukemia. 26:2390. 3. Heltemes-Harris LM, et al. 2012. Curr. Opin. Immunol. 24:146. 4. Heltemes-Harris LM, et al. 2011. Ann. N Y Acad. Sci. 1217:18. 5. Malin S, et al. 2010. Curr. Opin. Immunol. 22:168. 6. Xu J, et al. 2009. J. Neurosci. 29:2022.
Gene ID: 6776
UniProt: View information about STAT5 on UniProt.org
Clone: 9C8B50
Regulatory Status: RUO
Other Names: Signal transducer and activator of transcription 5A
Isotype: Mouse IgG2a, κ