Biolegend, 660402, Purified anti-CCL11 (Eotaxin) Antibody, 100μg

CCL11 selectively recruits eosinophils through binding to the chemokine receptor CCR3 during allergic response. CCL11 production is upregulated in response to several proimflammatory cytokines, such as IL-4, IL-13, TNF-α, and IFN-α, produced by mast cells and Th2 lymphocytes. CCL11 has been reported to stimulate eosinophil release from bone marrow, resulting in an elevated level of circulating eosinophils.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human CCL11 recombinant protein (Gly24-Pro97) expressed in E. coli.
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: Affinity purified.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2563471 (BioLegend Cat. No. 660402)
Structure: 74 amino acid, predicted molecular weight of approximately 12 kD.
Distribution: Secreted. Epithelial cells, smooth muscle cells, dendritic cells, and fibroblasts.
Interaction: Eosinophils
Ligand/Receptor: CCR3
Cell Type: Dendritic cells, Eosinophils, Epithelial cells, Fibroblasts
Biology Area: Angiogenesis, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Conroy DM, et al. 2001. Respir. Res. 2:150. 2. Levina V, et al. 2009. Clin. Cancer Res. 15:2647. 3. Salcedo R, et al. 2001. J. Immunol. 166:7571. 4. Kaehler J, et al. 2006. J. Investig. Med. 54:446. 5. Hadjicharalambous C, et al. 2004. J. Allergy Clin. Immunol. 113:657. 6. Crump MP, et al. 1998. J. Biol. Chem. 273:22471.
Gene ID: 6356
UniProt: View information about CCL11 on UniProt.org
Clone: L402H11
Regulatory Status: RUO
Other Names: Chemokine (C-C Motif) ligand 11, Eotaxin-1, Eosinophil chemotactic protein, Small-inducible cytokine A11
Isotype: Rat IgG2a, κ