Biolegend, 661202, Purified anti-CX3CL1 (Fractalkine) Antibody, 500μg

CX3CL1 is the only member of the CX3C chemokine family that is constitutively expressed in endothelial and epithelial cells, lymphocytes, neurons and microglial osteoblasts. CX3CL1 exists in two forms: as a membrane-anchored form of approximately 100 kD or as a shed 80-95 kD glycoprotein. The chemokine domain is roughly 8 kD, but it migrates at about 13 kD. Its membrane bound form promotes the firm adhesion of rolling leucocytes onto the vessel wall, while its soluble form serves as a potent chemoattractant for CX3CR1-expressing cells such as NK cells, CD14 + monocytes, cytotoxic effector T cells, B cells, neurons, microglia, smooth muscle cells, and tumor cells. The CX3CL1/CX3CR1 axis plays an important role in the pathogenesis of several inflammatory diseases such as allergic pathologies, rheumatoid arthritis, atherosclerosis and tumorigenesis.
500μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human CX3CL1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2563890 (BioLegend Cat. No. 661201) AB_2563891 (BioLegend Cat. No. 661202)
Structure: Chemokine, membrane bound form includes an extracellular NH2-terminal domain, a mucin-like stalk, a transmembrane α helix, and a short cytoplasmic tail. Full-length CX3CL1 contains over 300 amino acids and is highly glycosylated.
Distribution: Cell membrane, secreted.
Function: CX3CL1 has potent chemoattractant activity for T cells, monocytes, and natural killer cells, and the expression of CX3CL1 in activated endothelial cells promotes strong adhesion of those leukocytes. CXC3L1 is induced in endothelial cells by proinflammatory cytokines (TNF-α, IFN-γ, IL-1β) and LPS.
Interaction: Monocytic cells, smooth muscle cells, T cells, B cells, NK cells, microglia, and astrocytes.
Ligand/Receptor: CX3CR1
Cell Type: B cells
Biology Area: Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Ferretti E, et al. 2014. Mediators Inflamm. 2014:480941. 2. Apostolakis S, et al. 2013. Acta Pharmacol Sin. 34:1251. 3. Julia V, et al. 2012. Allergy. 67:1106. 4. Bazan JF, et al. 1997. Nature 6617:640. 5. Hundhausen C, et al. 2003. Blood 102:1186. 6. Sato E, et al. 2006. Ann. Rheum. Dis. 65:1257. 7. Jones B, et al. 2011. Front. Immunol. 2:82.
Gene ID: 6376
UniProt: View information about CX3CL1 on UniProt.org
Clone: L393H11
Regulatory Status: RUO
Other Names: Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1), ABCD-3, Fractalkine, Neurotactin (NTT, NTN), Small Inducible Cytokine Subfamily D (Cys-X3-Cys), Member-1 (SCYD1), CXC3C
Isotype: Mouse IgG2b, κ