Biolegend, 662202, Purified anti-EPO Antibody, 100μg

EPO is the major regulator of differentiation, proliferation, and survival of erythroid progenitors. Hypoxia induces erythropoiesis which causes cells to produce hypoxia inducible factor (HIF) that is directly involved in EPO expression. HIF-Prolyl hydroxylase (HIF-PH) is a sensor of levels of iron, oxygen, and metabolic activity. High levels of HIF protein induce EPO production in the kidneys and liver, and mobilize iron to support erythropoiesis. EPO has been used in the treatment of anemia associated with a number of conditions, such as chronic kidney disease, cancer patients on chemotherapy, and antiviral HIV therapy.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human EPO
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IP - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunoprecipitation. This antibody can be used at 1 - 5 µg/mL for immunoprecipitation. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2564245 (BioLegend Cat. No. 662202)
Structure: 165 amino acid protein with a predicted molecular weight of approximately 18 kD. It migrates at approximately 37-57 kD as a result of its glycosylation.
Distribution: EPO is primarily expressed in kidney (70-90% of the total EPO) and liver cells. EPO is expressed by hepatocytes, cortical interstitial fibroblasts in ischemic kidneys, astrocytes, and neurons under hypoxic conditions.
Function: EPO is the major regulator of differentiation, proliferation, and survival of erythroid progenitors. EPO expression is induced by hypoxia, HIF, and other transcription factors. EPO also plays a role in neuroprotection.
Interaction: Erythroid progenitor cells
Ligand/Receptor: EpoR (homodimer)
Cell Type: Fibroblasts, Neurons
Biology Area: Cell Biology, Signal Transduction
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Shoemaker CB, et al. 1986. Mol. Cell. Biol. 6:849. 2. McDonald JD, et al. 1986. Mol. Cell. Biol. 6:842. 3. Chin K, et al. 2000. Brain Res. Mol. Brain Res. 81:29. 4. Snow JW, et al. 2009. J. Biol. Chem. 284:29310. 5. Kassouf MT, et al. 2010. Genome Res. 2010:20:1064. 6. Paliege A, et al. 2010. Kidney Int. 77:312. 7. Sinclair AM, et al. 2010. Blood 115:4264. 8. Alnaeeli M, et al. 2012. Anat. Res. Int. 2012:953264.
Gene ID: 2056
UniProt: View information about EPO on UniProt.org
Clone: M0312E3
Regulatory Status: RUO
Other Names: Erythropoietin, MVCD2, EP, Epoetin
Isotype: Mouse IgG2b, κ