Biolegend, 662411, PE anti-IκB-α Antibody, 25tests

IκB-α is an inhibitory protein that binds to the NF-κB complex and retains this complex in the cytoplasm where it cannot activate transcription. Upon cellular stimulation, the IκB proteins are phosphorylated and degraded via the ubiquitin pathway allowing for nuclear translocation of NF-κB. The NF-κB complex can then bind DNA to activate gene expression of multiple proteins involved in inflammation, cellular proliferation, differentiation, and apoptosis.
25tests
Verified Reactivity: Human, Mouse, Rat, Hamster
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full length human IκB-α recombinant protein expressed in E. coli.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is ≤ 0.0075 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: This antibody weakly reacts with mouse and rat. Based on in-house testing, clone 3D6C02 does not block clone 25/IkBa/MAD-3, and only partially blocks L35A5 also raised against I?B-a.
Product Citations: Lu L, et al. 2022. Cancers (Basel). 14:. PubMed
RRID: AB_2783365 (BioLegend Cat. No. 662411) AB_2783366 (BioLegend Cat. No. 662412)
Structure: Inhibitory protein of the NF-κB complex that contains multiple ankyrin repeats.
Distribution: Cytoplasm
Function: Regulates transcriptional activity of NF-κB by binding the complex and trapping it in the cytoplasm. Serine phosphorylation of IκB targets it for destruction via the ubiquitination pathway, which allows the NF-κB complex to translocate to the nucleus.
Interaction: NFkB1, SUMO4, p53, ribosomal S6 kinase 1
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Signal Transduction
Molecular Family: Tumor Suppressors
Antigen References: 1. Baeuerle PA. 1998. Cell 95:729. 2. Hoffmann A, et al. 2002. Science 298:1241. 3. Ito CY, et al. 1995. Genomics 29:490.
Gene ID: 4792
UniProt: View information about IkappaB-alpha on UniProt.org
Clone: 3D6C02
Regulatory Status: RUO
Other Names: Nuclear factor of kappa light chain enhancer in B cells-inhibitor alpha, IKBA
Isotype: Mouse IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.