Biolegend, 662502, Purified anti-SCF Antibody, 100μg

Stem Cell Factor (SCF) is initially synthesized as membrane-bound forms of 248 or 220 amino acids, depending on the alternative splicing of exon 6. The 248 amino acid form contains a proteolytic cleavage site encoded by exon 6, and it is cleaved from the cell to release an active soluble protein of 165 amino acid residues. Soluble SCF is glycosylated at both N-linked and O-linked sites. MMP-9 plays a physiological role in SCF release from the membrane, and this action plays a significant role in differentiating and mobilizing stem and progenitor cells from the bone marrow. SCF increases the proliferation of myeloid and lymphoid hematopoietic progenitors in bone marrow cultures. SCF/c-kit interaction in mast cells results in mast cell degranulation that induces the release of mediators, such as histamine and inflammatory cytokines and chemokines. Also, activation of c-kit in dendritic cells regulates T helper cell differentiation and allergic asthma. In addition, SCF plays an important role in revascularization of ischemic limbs. Ischemia induces plasma elevation of SCF, thrombopoietin (TPO), erythropoietin (EPO), and GM-CSF albeit at lower levels. SCF and TPO induce the release of CXCL12 from platelets, which increase CXCL12 levels in plasma. This results in an extensive mobilization of CXCR4 + VEGFR1 + cells (hemangiocytes) that accelerate revascularization of the ischemic limbs. SCF binds to its receptor c-kit, which belongs to the type III tyrosine kinase family whose members include receptors for M-CSF and PDGF.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human SCF
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2564302 (BioLegend Cat. No. 662502)
Structure: Homodimer (non-covalently linked).
Distribution: Cytoplasm and cytoskeleton.
Function: Stem Cell Factor (SCF) plays a role in the regulation of cell proliferation, melanocyte differentiation, innate immune response, hematopoiesis, stem cell maintenance, mast cell development, migration, and function. Binding of SCF to c-Kit leads to the activation of PI3 kinase, Src kinase, and several signaling pathways including JAK/STAT pathway (activation of STATs) and Ras/Erk pathway (transmit signals through adaptor protein Grb2).
Interaction: Mast cells, natural killer cells, dendritic cells, eosinophils, epithelial cells, endothelial cells, melanocytes, germ cells, cholangiocytes, platelets, myeloid leukaemia cells, and intestinal cells of Cajal.
Ligand/Receptor: c-kit (CD117)
Biology Area: Angiogenesis, Cell Biology, Immunology, Signal Transduction, Stem Cells
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: 1. Lu HS, et al. 1996. J. Biol. Chem. 271:11309.2. Heissig B, et al. 2002. Cell 109:625.3. Ronnstrand L. 2004. Cell Mol. Life Sci. 61:19-20.4. Jin DK, et al. 2006. Nat. Med. 12:557.5. Krishnamoorthy N, et al. 2008. Nat. Med. 14:565.6. Ray P, et al. 2010. Ann. N. Y. Acad. Sci. 1183:104.
Gene ID: 4254
UniProt: View information about SCF on UniProt.org
Clone: L061D3
Regulatory Status: RUO
Other Names: Stem cell factor, KIT-ligand, Kitl, KL-1, KL1, mast cell grow factor (MGF), steel factor (SF), FPH2, SHEP7
Isotype: Mouse IgG1, κ