Biolegend, 663305, Purified anti-, 25μg

Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and then are released. This sigma factor is responsible for the expression of enzymes involved in arginine catabolism. The open complex (Sigma 54 and core RNA polymerase) serves as the receptor for the receipt of the melting signal from the remotely bound activator protein glnG (ntrC).
25μg
Verified Reactivity: E. coli
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Previously item# W0005 from Neoclone. Clone 6RN3 recognizes epitope 88-137. Additional reported applications (for the relevant formats) include: functional assay.1
Application References(PubMed link indicates BioLegend citation): Zhao K, et al. 2010. Nucleic Acids Res. 38:1273. (FA)
RRID: AB_2820108 (BioLegend Cat. No. 663305) AB_2820109 (BioLegend Cat. No. 663306)
Structure: Sigma 54 (σ54) is a 54 kD transcription factor of E. coli RNA polymerase. Observed molecular weight is around 70 kD.
Biology Area: Cell Biology, Transcription Factors
Molecular Family: Nuclear Markers
Gene ID: 947714
UniProt: View information about Sigma 54 on UniProt.org
Clone: 6RN3
Regulatory Status: RUO
Other Names: rpoN, glnF, ntrA, RNA Polymerase
Isotype: Mouse IgG2a, κ