Biolegend, 663907, Direct-Blot™ HRP anti-, 100microl

RNA polymerase (RNAP or RNApol) is an enzyme that produces primary transcript RNA. The β subunit is the second-largest subunit, and is encoded by the roB gene. RNA polymerase β is a subunit which, along wtih α (rpoC), limits core polymerase expression.
100microl
Verified Reactivity: E. coli
Reported Reactivity: Other species
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Purified recombinant E. coli RNA polymerase β subunit.
Formulation: This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation: The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:4000. The optimal dilution should be determined by titration for each individual assay of interest. 25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.
Application Notes: Clone 8RB13 is extremely useful for detecting or purifying core RNAP from a broad range of bacteria. The purified polymerase is highly active for in vitro transcription. Additional reported applications (for the relevant formats) include: immunoprecipitation3. The epitope of clone 8RB13 has been mapped to the sequence PEEKLLRAIFGEKAS3.
Application References(PubMed link indicates BioLegend citation): Bergendahl V, et al. 2003. Protein Expr. Purif. 31:155. Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304. Stalder, ES. et al. 2011. Protein Expr. Purif. 77(1):26-33. (Epitope, ELISA, IP, WB)
RRID: AB_2629625 (BioLegend Cat. No. 663907)
Structure: 1252 amino acids with a predicted molecular weight of approximately 150 kD.
Distribution: Bacteria.
Function: Transcriptional regulator in bacteria.
Biology Area: Cell Biology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Thompson NE, et al. 1992. Biochemistry 31:7003. 2. Jerald Hurwitz, et al. 2005. J. Biol. Chem. 280:42477. 3. Hirata A, et al. 2008. Nature. 451:851. 4. Bergendahl IV, et al. 2003. Protein Expr. Purif. 31:155. 5. Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304.
Gene ID: 948488
UniProt: View information about RNA Polymerase beta on UniProt.org
Clone: 8RB13
Regulatory Status: RUO
Other Names: RNAP subunit beta, rpoB, groN, nitB, rif, ron, stl, stv, tabD, RNA polymerase subunit beta, Transcriptase subunit beta
Isotype: Mouse IgG2b, κ