Biolegend, 664803, Direct-Blot™ HRP anti-β-actin Antibody, 25microl

β-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting).
25microl
Verified Reactivity: Human, Mouse, Rat, Zebrafish, Drosophila
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human ACTB peptide (1-13 a.a.).
Formulation: This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation: The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.001 - 0.01 µg per ml (1:10,000 - 1:100,000 dilution). It is recommended that the reagent be titrated for optimal performance for each application. 25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.
Application Notes: Reactivity to Drosophila was only verified with the purified format.
Product Citations: Altemus J, et al. 2022. J Cell Physiol. 237:3001. PubMed Li Y, et al. 2020. Stem Cell Res Ther. 0.815972222. PubMed Sripathi SR, et al. 2021. Mol Cell Proteomics. 20:100131. PubMed Wijeratne TU, et al. 2022. J Biol Chem. :102319. PubMed
RRID: AB_2810693 (BioLegend Cat. No. 664803) AB_2728496 (BioLegend Cat. No. 664804)
Structure: 375 amino acids with a predicted molecular weight of 42 kD.
Distribution: Cytoplasm
Function: Actins are highly conserved proteins that are involved in cell motility.
Interaction: Interacts with CPNE1 and CPNE4, ERBB2, GCSAM, TBC1D21.
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family: Microfilaments
Antigen References: 1. Hanukoglu I, et al. 1983. J. Mol. Biol. 163:673. 2. Nakajima-Iijima S, et al. 1985. Proc. Natl. Acad. Sci. 82:6133. 3. Ponte P, et al. 1984. Nucleic Acids Res. 12:1687.
Gene ID: 60
UniProt: View information about beta-actin on UniProt.org
Clone: W16197A
Regulatory Status: RUO
Other Names: ACTB, Actin, cytoplasmic 1, Beta-actin
Isotype: Rat IgG2a, κ