Biolegend, 666202, Purified anti-eIF4A1 Antibody, 100μg

EIF4A1 is an ATP-dependent RNA helicase. It can form the eIF4F complex, which is involved in translation initiation by binding with the 5' m 7 GTP cap of the mRNA. It could unwind mRNA secondary structure at the 5’ UTR region and lead to the small ribosomal subunit binding and followed scanning of the initiator codon.
100μg
Verified Reactivity: Human, Mouse, Rat, Hamster
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Human EIF4A1 Peptide (3-19 a.a.) conjugated to KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedICC, IP, IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.025 - 0.5 µg/mL (1:1000 - 1:20000 dilution). For immunocytochemistry, a concentration range of 0.5 - 2.0 μg/mL (1:250 - 1:1000 dilution) is recommended. For immunoprecipitation, the suggested use of this reagent is 5.0 - 15 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 5.0 - 10 µg/mL (1:50 - 1:100 dilution) is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2783367 (BioLegend Cat. No. 666201) AB_2783368 (BioLegend Cat. No. 666202)
Distribution: Cytoplasm
Function: Translation initiation
Interaction: eIF4 complex
Biology Area: Cell Biology, Cell Proliferation and Viability, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation, Protein Synthesis, Signal Transduction
Gene ID: 1973
UniProt: View information about eIF4A1 on UniProt.org
Clone: W17130C
Regulatory Status: RUO
Other Names: EIF4A1, Eukaryotic Translation Initiation Factor 4A1
Isotype: Rat IgG2a, κ