Biolegend, 666409, Alexa Fluor® 647 anti-STAT1 Phospho (Tyr701) Antibody, 25tests

STAT1, also known as signal transduction and activator of transcription 1, is a ubiquitously expressed cytoplasmic protein and is activated in response to cytokine signaling, including IFN-α, IFN-γ, EGF, PDGF, and IL-6. Upon activation, STAT1 is phosphorylated at Tyrosine 701 (Tyr701) by receptor-associated kinases, including JAK1, JAK2, and TYK2. This results in STAT1 dimerization and subsequent translocation to the nucleus, where it functions as a transcriptional activator. STAT1 is involved in IFN-mediated immune responses, and STAT1-deficient mice are highly sensitive to bacterial and viral infections.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: Clone A17012A recognizes STAT1 phosphorylated at Tyrosine 701 (Tyr701). When using this clone for ICC, we recommend using methanol to permeabilize fixed cells.
Product Citations: Li Z, et al. 2024. Front Immunol. 1455019:15. PubMed
RRID: AB_2814502 (BioLegend Cat. No. 666409) AB_2814503 (BioLegend Cat. No. 666410)
Structure: Two isoforms of STAT1 exist as a result of alternative splicing. Isoform α is a 750 amino acid protein with a predicted molecular weight of 87 kD; the β isoform is a 712 amino acid protein with a predicted molecular weight of 83 kD.
Distribution: Ubiquitous tissue expression, nucleoplasmic-cytosolic distribution
Function: Immune response activation, cell signaling
Interaction: STAT1, STAT2, IRF9, ERBB4, JAK1, JAK2, TYK2, TNK1, SHP2
Biology Area: Cell Biology
Molecular Family: Nuclear Markers, Phospho-Proteins
Antigen References: Moretti S, et al. 2017. J. Biol. Chem. 292: 1785. Wei J, et al. 2015. J. Immunol. 195: 2870. Sung PS, et al. 2015. Proc. Natl. Acad. Sci. USA. 112: 10443 Ooi EL, et al. 2014. Proc. Natl. Acad. Sci. USA. 111: 1909. Wu TR, et al. 2002. J. Biol. Chem. 277: 47572. Horvath, et al. 1996. J. Virol. 70: 647. Haque SJ, et al. 1995. J. Biol. Chem. 270: 25709.
Gene ID: 6772
UniProt: View information about STAT1 Phospho Tyr701 on UniProt.org
Clone: A17012A
Regulatory Status: RUO
Other Names: Signal Transduced Activator of Transcription 1, Transcription Factor ISGF-3 components p91/p84
Isotype: Mouse IgG1, κ